石蒜碱通过调控Wnt/β-catenin信号通路抑制宫颈癌Caski细胞侵袭迁移作用的研究  

Lycorine Inhibits the Invasion and Migration of Cervical Cancer Caski Cells By Regulating Wnt/β-catenin Signaling Pathway

作　　者：周欣[1] ZHOU Xin(Department of Gynecology,Jiamusi Maternal and Child Health Hospital,Jiamusi 154000,Heilongjiang,China)

机构地区：[1]佳木斯市妇幼保健院妇科,黑龙江佳木斯154000

出　　处：《系统医学》2024年第15期33-36,共4页Systems Medicine

基　　金：黑龙江省卫生健康委科研立项(20220505011163)。

摘　　要：目的 探讨石蒜碱对宫颈癌Caski细胞侵袭迁移作用的影响以及可能的作用机制。方法 选取2023年6—12月佳木斯大学基础医学院实验室培养的宫颈癌Caski细胞接种于96孔培养皿中,贴壁2 h后更换无血清1640培养液,将无处理Caski细胞作为对照组,将加入2、4、8 ug/mL石蒜碱的Caski细胞作为低浓度实验组、中浓度实验组和高浓度实验组,每组设置5个复孔。在培育12、24、48 h后,检测不同浓度石蒜碱对Caski细胞增值抑制作用,及对Caski细胞侵袭迁移的影响,RT-PCR检测c-Myc、G1/S-特异性周期蛋白-D1(Cyclin D1)mRNA的表达水平,western blot检测β-catenin及c-Myc、Cyclin D1蛋白表达水平。结果 与对照组比较,石蒜碱明显抑制了Caski细胞增殖,且呈时间浓度依赖性。对照组Caski细胞侵袭迁移细胞数分别为(610.20±36.63)个、(220.00±22.26)个,高于实验组的(367.60±28.14)个、(60.40±9.65)个,差异有统计学意义(t=11.744、14.709,P均<0.05)。与对照组比较,实验组β-catenin、c-Myc、Cyclin D1的mRNA、蛋白表达均较低,差异有统计学意义(P均<0.05)。结论 石蒜碱可能通过靶向Wnt/β-catenin信号通路抑制Caski细胞增值,进而抑制宫颈癌迁移和侵袭。Objective To investigate the effect of lycorine on invasion and migration of Caski cells of cervical cancer and its possible mechanism.Methods Cervical cancer Caski cells cultured in the laboratory of School of Basic Medi-cine of Jimusi University from June to December 2023 were selected and inoculated into 96-well petri dishes.After being attached to the wall for 2 h,the serum-free 1640 culture medium was replaced.The untreated Caski cells were used as the control group.Caski cells added with 2,4 and 8 ug/mL lycorine were divided into low concentration ex-perimental group,medium concentration experimental group and high concentration experimental group,and 5 com-pound pores were set in each group.After incubation for 12,24 and 48 h,the inhibitory effect of lycorine on prolifera-tion of Caski cells and its effect on invasion and migration of Caski cells were detected.The mRNA expression levels of c-Myc and G1/S-specific Cyclin D1(Cyclin D1)were detected by RT-PCR.The expression levels ofβ-catenin,c-Myc and Cyclin D1 were detected by western blot.Results Compared with the control group,lycorine significantly inhibited the proliferation of Caski cells in a time-concentration dependent manner.The invasion and migration num-bers of Caski cells in the control group were 610.20±36.63 and 220.00±22.26,which were higher than 367.60±28.14 and 60.40±9.65 in the experimental group,and the differences were statistically significant(t=11.744,14.709,both P<0.05).Compared with control group,mRNA and protein expressions ofβ-catenin,c-Myc and Cyclin D1 in experimen-tal group were lower,and the differences were statistically significant(all P<0.05).Conclusion Lycorine may inhibit the proliferation of Caski cells by targeting Wnt/β-catenin signaling pathway.

关 键 词：石蒜碱 WNT/Β-CATENIN CASKI细胞 侵袭迁移 

分 类 号：R4[医药卫生—临床医学]