探讨TRUST与TP-ELISA对梅毒患者的诊断价值  

To Explore the Diagnostic Value of TRUST and TP-ELISA in Syphilis Diagnosis

作　　者：任勇[1] 杨云敏 REN Yong;YANG Yunmin(Department of Medical Laboratory,Xingyi People's Hospital of Guizhou Province,Xingyi 562400,Guizhou,China)

机构地区：[1]贵州省兴义市人民医院医学检验科,贵州兴义562400

出　　处：《系统医学》2024年第15期98-100,共3页Systems Medicine

摘　　要：目的 探讨甲苯胺红不加热血清试验(toludine red unheated serum test, TRUST)、梅毒螺旋体-酶联免疫吸附试验(treponema pallidum-enzyme linked immunosorbent assay, TP-ELISA)检测方法在梅毒诊断中的诊断价值。方法 非随机选取2022年1月—2023年10月兴义市人民医院收治的86例疑似梅毒患者为研究对象,所有患者均完成TRUST与TP-ELISA检测,并将梅毒螺旋体明胶颗粒凝集试验(treponema palli-dum particle agglutination, TPPA)检测作为金标准,分析TRUST与TP-ELISA的诊断价值。结果 86例疑诊梅毒患者中,61例患者确诊为梅毒,患病率为70.93%(61/86)。TRUST检查的阳性率为59.30%(51/86),阴性率为40.70%(35/86);TP-ELISA检查的阳性率为67.44%(58/86),阳性率为32.56%(28/86)。TP-ELISA的敏感度为93.44%(57/61),高于TRUST检查的80.33%(49/61),差异有统计学意义(χ^(2)=4.604,P=0.032);且TP-ELISA准确度为94.19%(81/86),高于TRUST的83.72%(72/86),差异有统计学意义(χ^(2)=4.793,P=0.029)。结论 在梅毒诊断中,TP-ELISA的敏感度、准确度高于TRUST,但筛查过程仍存在假阴性风险,临床应综合考虑患者的危险因素筛查结果与其他临床指标。Objective To explore the diagnostic value of toludine red unheated serum test(TRUST)and treponema pallidum-enzyme linked immunosorbent assay(TP-ELISA)in the diagnosis of syphilis.Methods Eighty-six patients with suspected syphilis admitted to Xingyi People′s Hospital from January 2022 to October 2023 were non-randomly selected as the research objects.All patients completed TRUST and TP-ELISA tests.The treponema palli-dum par-ticle agglutination(TPPA)test was used as the gold standard to analyze the diagnostic value of TRUST and TP-ELISA.Results Among the eighty-six patients with suspected syphilis,eighty-one patients were diagnosed with syphi-lis,and the prevalence rate was 70.93%(61/86).The positive rate of TRUST was 59.30%(51/86),and the negative rate was 40.70%(35/86).The positive rate of TP-ELISA was 67.44%(58/86),and the positive rate was 32.56%(28/86).The sensitivity of TP-ELISA was 93.44%(57/61),which was higher than 80.33%(49/61)of TRUST,and the dif-ference was statistically significant(χ^(2)=4.604,P=0.032).The accuracy of TP-ELISA was 94.19%(81/86),which was higher than 83.72%(72/86)of TRUST,and the difference was statistically significant(χ^(2)=4.793,P=0.029).Conclu-sion In the diagnosis of syphilis,the sensitivity and accuracy of TP-ELISA are higher than those of TRUST,but there is still a risk of false negative in the screening process.Clinically,the screening results of risk factors and other clini-cal indexs of patients should be considered comprehensively.

关 键 词：梅毒 梅毒螺旋体-酶联免疫吸附试验检测 甲苯胺红不加热血清检测 诊断效能 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]