黄芩苷调节线粒体动力学平衡后减缓小鼠骨骼肌缺血再灌注损伤的研究  

Effects of baicalin in retarding mice skeletal muscle ischemia-reperfusion injury after regulating mitochondrial dynamic balance

作　　者：张永春 孙林 徐凯 陈绪龙 李昊 张永创 杨清滔 ZHANG Yongchun;SUN Lin;XU Kai;CHEN Xulong;LI Hao;ZHANG Yongchuang;YANG Qingtao(Second Department of Surgery;Department of Medical Administration,Hospital of Armed Police Guizhou Provincial Corps,Guiyang,Guizhou 550005,China;Department of Urologic Surgery,Affiliated Hospital of Guizhou Medical University,Guiyang,Guizhou 550004,China)

机构地区：[1]武警贵州省总队医院外二科,贵州贵阳550005 [2]武警贵州省总队医院卫勤处,贵州贵阳550005 [3]贵州医科大学附属医院泌尿外科,贵州贵阳550004

出　　处：《检验医学与临床》2024年第19期2835-2840,共6页Laboratory Medicine and Clinic

基　　金：贵州省卫生健康委员会科学技术基金项目(gzwkj2021-199)。

摘　　要：目的研究黄芩苷对小鼠骨骼肌缺血再灌注损伤是否具有抑制作用,该抑制作用是否通过调节线粒体动力学平衡来实现。方法随机将30只昆明小鼠分为空白对照组、缺血复供组及低、中、高剂量黄芩苷干预组。除空白对照组外,其余各组小鼠构建双下肢缺血再灌注损伤模型,并使用不同剂量黄芩苷溶液腹腔注射干预,缺血复供组使用生理盐水替代。采用苏木素-伊红(HE)染色检测各组骨骼肌损伤情况,检测各组骨骼肌组织线粒体分裂蛋白1(FIS1)、线粒体动力相关蛋白1(DRP1)、线粒体融合蛋白(Mfn)1、Mfn2的基因和蛋白表达水平,同时检测各组骨骼肌组织超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)水平。结果小鼠双下肢缺血再灌注损伤后,下肢骨骼肌出现明显损伤和炎症浸润。与空白对照组相比,其他各组骨骼肌组织FIS1、DRP1的基因和蛋白表达及MDA水平均明显升高(P<0.05),Mfn1、Mfn2的基因和蛋白表达及GSH、SOD水平均明显降低(P<0.05)。给予3种剂量黄芩苷溶液干预小鼠模型后,与缺血复供组比较,高剂量黄芩苷干预组DRP1基因表达明显下降(P<0.05),中、高剂量黄芩苷干预组的DRP1蛋白表达均明显下降(P<0.05),高剂量黄芩苷干预组GSH、SOD水平均明显升高(P<0.05),高剂量黄芩苷干预组MDA水平明显下降(P<0.05)。不同剂量黄芩苷干预组的FIS1基因、蛋白表达比较,差异均无统计学意义(P>0.05),不同剂量黄芩苷干预组Mfn1、Mfn2的基因及蛋白表达出现少量升高但差异均无统计学意义(P>0.05)。结论小鼠下肢骨骼肌缺血再灌注损伤后,下肢肌群发生氧化应激损伤,线粒体动力学失衡。黄芩苷对骨骼肌缺血再灌注损伤的抑制作用可能与减轻氧化应激损伤、抑制DRP1的表达有关。Objective To investigate whether baicalin having the inhibitory effect on mice skeletal muscle ischemia-reperfusion injury and whether the inhibitory effect being achieved by regulating mitochondrial dynamic balance.Methods Thirty Kunming mouse models were divided into the blank control group,ischemia-reperfusion group,low,medium and high-dose baicalin intervention groups.Except for the blank control group,the mice in the other groups were constructed the bilateral lower limb ischemia-reperfusion injury model,and different doses of baicalin solution were intraperitoneally injected for intervention.The ischemia-reperfusion group was replaced with physiological saline.The HE staining was used to detect the pathological damage of skeletal muscle,and detected the gene and protein expression levels of mitochondrial fission protein 1(FIS1),mitochondrial dynamics related protein 1(DRP1),mitochondrial fusion protein(Mfn)1,and Mfn2 in skeletal muscle tissues of each group.At the same time,the levels of superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione(GSH)in skeletal muscle tissues of each group were detected.Results After ischemia-reperfusion injury in both lower limbs of mice,the skeletal muscles of the lower limbs developed significant damage and inflammatory cell infiltration;compared with the blank control group,the gene and protein expression of FIS1 and DRP1,as well as the levels of MDA in skeletal muscle tissues of other groups were significantly increased(P<0.05);the gene and protein expression of Mfn1 and Mfn2 as well as GSH and SOD levels were significantly reduced(P<0.05).After administering three doses of baicalin solution to intervene in the mouse model,compared with the ischemia-reperfusion group,the DRP1 gene expression in the high-dose baicalin intervention group was significantly decreased(P<0.05),while the DRP1 protein expression in the medium and high-dose baicalin intervention groups was significantly decreased(P<0.05),the levels of GSH and SOD in the high-dose baicalin intervention group

关 键 词：黄芩苷 线粒体 动力学平衡 骨骼肌 缺血再灌注损伤 

分 类 号：R642[医药卫生—外科学] R446[医药卫生—临床医学]