小鼠囊胚培养液用于染色体筛查的研究  

Study on of mouse blastocyst culture medium for chromosome screening

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作  者:侯东霞 诺明途 黄艳 马科 王晓华 王瑞 HOU Dongxia;NUO Mingtu;HUANG Yan;MA Ke;WANG Xiaohua;WANG Rui(Inner Mongolia Autonomous Region Maternal and Child Health Care Hospital,Hohhot 010010,China)

机构地区:[1]内蒙古自治区妇幼保健院,内蒙古呼和浩特010010

出  处:《包头医学院学报》2024年第9期18-23,共6页Journal of Baotou Medical College

基  金:内蒙古自治区自然科学基金项目(2020MS08202);内蒙古自治区关键技术攻关计划项目(2019GG103);内蒙古自治区第十二批“草原英才”工程高层次培养人才项目。

摘  要:目的:利用小鼠囊胚培养液探讨无创胚胎植入前非整倍体筛查(niPGT-A)的可行性,为优化和发展无创胚胎植入前遗传学筛查技术提供理论依据和数据支持。方法:本研究共收集11枚小鼠囊胚,同一囊胚分别取少量滋养外胚层细胞(TE)作为金标准、内细胞团(ICM)细胞作为阳性对照、胚胎培养液(BCM)作为待验证样本。所有胚胎样本基于全基因组测序数据进行染色体状态分析,并比较同一胚胎不同样本间染色体倍性一致性。结果:在11枚小鼠囊胚中,BCM、TE和ICM的PGT-A检出率分别为82.8%(9/11)、72.7%(8/11)和100%(11/11)。BCM与TE、BCM与ICM及TE与ICM的染色体一致性分别为85.7%(6/7),88.9%(8/9),87.5%(7/9),任意两组间差异无统计学意义(P>0.05)。结论:在小鼠模型中,相比于同期活检的滋养外胚层细胞,利用囊胚培养液中的游离DNA行无创胚胎植入前非整倍体检测可获得更高的检出率和更为准确的染色体检测结果。Objective:To explore the feasibility of non-invasive preimplantation genetic testing for aneuploidy(niPGT-A)using mouse blastocyst culture medium,and to provide theoretical basis and data support for optimizing and developing non-invasive preimplantation genetic screening technology.Methods:A total of 11 mouse blastocysts were collected.A small amount of trophectoderm cells(TE)were taken as the gold standard,inner cell mass(ICM)cells as the positive control,and blastocyst culture medium(BCM)as the sample to be verified.All embryo samples were analyzed for chromosome status based on whole genome sequencing data,and the chromosome ploidy consistency between different samples of the same embryo was compared.Results:In 11 mouse blastocysts,the detection rates of PGT-A in BCM,TE and ICM were 82.8%(9/11),72.7%(8/11)and 100%(11/11),respectively.The chromosome consistency between BCM and TE,BCM and ICM,TE and ICM was 85.7%(6/7),88.9%(8/9),87.5%(7/9),respectively.There was no significant difference between any two groups(P>0.05).Conclusion:In mouse model,noninvasive preimplantation genetic testing for aneuploidy using cell free DNA in blastocyst culture medium can obtain higher detection rate and more accurate chromosome detection results than trophoblastic ectoderm cells biopsy at the same time.

关 键 词:小鼠胚胎 无创胚胎植入前非整倍体筛查 囊胚培养液 滋养层活检 内细胞团 

分 类 号:R714.8[医药卫生—妇产科学]

 

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