暖宫七味丸对环磷酰胺诱导的卵巢早衰小鼠卵巢功能的影响  

Effect of Nuangong Qiwei Pill on ovarian function in mice with premature ovarian failure induced by cyclophosphamide

作　　者：王宁 何金英 WANG Ning;HE Jinying(Graduate School,Baotou Medical College,Baotou 014000,China;Department of Reproductive Medicine,Inner Mongolia Autonomous Region People's Hospital)

机构地区：[1]包头医学院研究生院,内蒙古包头014040 [2]内蒙古自治区人民医院生殖医学科

出　　处：《包头医学院学报》2024年第9期47-51,共5页Journal of Baotou Medical College

摘　　要：目的:观察蒙药暖宫七味丸对环磷酰胺诱导的卵巢早衰小鼠是否有保护作用。方法:将动情周期正常的雌性小鼠随机分为5组:空白对照组,环磷酰胺组,暖宫七味丸低剂量组、中剂量组和高剂量组。除空白对照组以外,其余各组小鼠腹腔注射环磷酰胺制备卵巢早衰模型[首剂量50 mg/kg,之后按8 mg/(kg·d),共14 d],造模结束后空白对照组和环磷酰胺组给予0.1 mL生理盐水灌胃,暖宫七味丸低、中、高剂量组分别予0.25、0.5、0.8 g/kg灌胃,连续14 d。每日监测各组小鼠的体质量变化及动情周期改变,干预结束后采集小鼠血清检测抗苗勒氏管激素(AMH)、雌二醇(E2)、卵泡刺激素(FSH)水平,取卵巢组织切片染色后分析。结果:与空白对照组比较,环磷酰胺组小鼠体质量下降,动情周期消失,血清E2和AMH水平显著降低,FSH水平显著提高(P<0.01),卵巢切片见闭锁卵泡明显增加,间质纤维化。与环磷酰胺组比较,暖宫七味丸各剂量组小鼠体质量无明显差异;中、高剂量组小鼠动情周期明显恢复;而高剂量组小鼠的血清E2水平显著提高(P<0.05),AMH及FSH水平未见明显变化(P>0.05),卵巢组织及结构有一定程度改善。结论:蒙药暖宫七味丸能在一定程度上改善环磷酰胺所致损伤的卵巢功能,采用高剂量治疗14 d明显恢复动情周期,改善血清激素水平,修复卵巢受损组织,对卵巢早衰起一定治疗作用。Objective:To observe the protective effect of Mongolian medicine Nuangong Qiwei Pill on cyclophosphamide-induced premature ovarian failure mice.Methods:Female mice with normal estrous cycle were randomly divided into 5 groups:blank control group,cyclophosphamide group,low dose group,medium dose group and high dose group of Nuangong Qiwei Pill.Except for the blank control group,the mice in the other groups were intraperitoneally injected with cyclophosphamide to prepare the premature ovarian failure model(the first dose was 50 mg/kg,followed by 8 mg/kg/d for 14 days).After the modeling,the blank control group and the cyclophosphamide group were given 0.1 ml normal saline by gavage,and the low,medium and high dose groups of Nuangong Qiwei Pill were given 0.25,0.5 and 0.8 g/kg by gavage for 14 days.The changes of body weight and estrous cycle of mice in each group were monitored daily.After the intervention,the serum of mice was collected to detect the levels of anti-mullerian hormone(AMH),estradiol(E2)and follicle stimulating hormone(FSH),and the ovarian tissue sections were stained and analyzed.Results:Compared with the blank control group,the body weight of the mice in the cyclophosphamide group decreased,the estrous cycle disappeared,the serum E2 and AMH levels decreased significantly,and the FSH level increased significantly(P<0.01).Ovarian sections showed a significant increase in atretic follicles and interstitial fibrosis.Compared with the cyclophosphamide group,there was no significant difference in body weight of mice in each dose group of Nuangong Qiwei Pills;the estrous cycle of mice in the medium and high dose groups was significantly restored;the serum E2 level of mice in the high dose group was significantly increased(P<0.05),the AMH and FSH levels did not change significantly(P>0.05),and the ovarian tissue and structure were improved to a certain extent.Conclusion:Mongolian medicine Nuangong Qiwei Pill can improve the ovarian function of cyclophosphamide-induced model to a certain extent.High dose tr

关 键 词：蒙药 暖宫七味丸 卵巢早衰 环磷酰胺 小鼠 

分 类 号：R285.5[医药卫生—中药学]