近年来我国西部地区小反刍兽疫病毒N、F基因的遗传演化分析  

Genetic Evolution Analysis on N and F Genes of Peste des petits Ruminants Virus in Western China

作　　者：徐蛟 苏娜 王英丽[1] 刘珊[1] 张永强[1] 邹艳丽[1] 任炜杰[1] 包静月[1] 王志亮[1] Xu Jiao;Su Na;Wang Yingli;Liu Shan;Zhang Yongqiang;Zou Yanli;Ren Weije;Bao Jingyue;Wang Zhiliang(China Animal Health and Epidemiology Center,Qingdao,266032,Shandong,China;Qingdao Agricultural University,Qingdao 266109,Shandong,China)

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]青岛农业大学,山东青岛266109

出　　处：《中国动物检疫》2024年第9期1-8,共8页China Animal Health Inspection

基　　金：“十四五”国家重点研发计划项目(2022YFD1800500)。

摘　　要：为了解近年来我国西部地区小反刍兽疫病毒(PPRV)的遗传演化情况,收集2020—2024年新疆、西藏、青海部分地区报告的5起小反刍兽疫(PPR)疫情发病动物的组织和拭子样品,利用荧光RT-PCR对PPRV进行初步检测,并对PPRV的N和F基因进行同源性比对和系统进化树绘制。结果显示:本研究检出的PPRV阳性样品均属于谱系IV型;与2014年以来国内的PPRV流行毒株以及蒙古国野生动物PPRV毒株亲缘关系较近,与除蒙古国外的其他国家流行株同源性较低;从野生岩羊和家羊中检出的PPRV阳性样品在进化树上没有显著差别。结果说明:2020—2024年我国西部地区发生的5起PPR疫情可能均与国内的流行株相关,病毒从周边国家传入的可能性较小,病毒暂未发生明显变化,家羊与野羊感染毒株具有相似的遗传来源。In order to investigate the genetic evolution of peste des petits ruminants virusPPRVin western China in recent years,tissue and swab samples were collected from infected animals in 5 outbreaks of peste des petits ruminant(PPR)reported in Xinjiang Uygur Autonomous Region,Xizang Autonomous Region,Qinghai Province from 2020 to 2024 to detect PPRV by fluorescent RT-PCR,followed by homology comparison of N and F genes of the virus and construction of a phylogenetic tree.The results showed that all the positive samples belonged to lineage IV,close to the PPRV strain in China since 2014 and that derived from wildlife in Mongolia,and less homologous to the strains in other countries except Mongolia;no significant difference was observed among the positive samples from wild blue sheep and domestic sheep in genetic evolution according to the evolutionary tree.In conclusion,the 5 PPR outbreaks in western China from 2020 to 2024 might be associated with domestic strains,with little probability of introduction from neighbouring countries,and the virus had not changed significantly,the strains isolated from domestic and wild sheep were with similar genetic origin.

关 键 词：小反刍兽疫 疫情 诊断 遗传演化 

分 类 号：S851.3[农业科学—预防兽医学]