布洛芬对海洋青鳉鱼生命初期毒性效应  

Toxic effects of ibuprofen on early life stages of marine medaka(Oryzias Melastigma)

作　　者：马正茁 梁川 孙哲诚 陈杨 吴懂 徐炎华 刘志英 MA Zhengzhuo;LIANG Chuan;SUN Zhecheng;CHEN Yang;WU Dong;XU Yanhua;LIU Zhiying(School of Environmental Science and Engineering,Nanjing Tech University,Nanjing,211816,China)

机构地区：[1]南京工业大学环境科学与工程学院,南京211816

出　　处：《环境化学》2024年第9期2934-2943,共10页Environmental Chemistry

基　　金：国家十三五重点研发课题(2017YFB0602500)资助.

摘　　要：为探究布洛芬(Ibuprofen,IBP)对海洋青鳉鱼(Oryzias melastigma)早期生命阶段的毒性效应,将海洋青鳉鱼胚胎在不同浓度(0、1、10、100、1000μg·L^(-1))IBP溶液中暴露至孵化后的第30天(未孵化胚胎暴露20 d).观察统计胚胎的孵化率、孵化时间、心率、仔鱼30 d存活率以及体长体重等发育参数,并检测胚胎的抗氧化酶(超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽巯基转移酶(Glutathione S-transferase,GST))活性、抗氧化剂(还原型谷胱甘肽(glutathione,GSH))、氧化产物(丙二醛(malonic dialdehyde,MDA))浓度和神经递质降解酶(乙酰胆碱酯酶(acetylcholinesterase,AChE))活性变化,以评估IBP对海洋青鳉鱼早期生命阶段的发育毒性、抗氧化系统以及神经递质系统的影响.结果表明,1μg·L^(-1)IBP对海洋青鳉鱼胚胎及仔鱼不存在发育毒性,其余各浓度导致胚胎孵化率、孵化时间、心率和仔鱼存活率降低,所有浓度下仔鱼体长体重均未变化;在第5天时,随浓度升高,SOD活性和GSH含量呈下降趋势,CAT、GST活性和MDA含量先上升后下降,第8天时各指标趋于正常;IBP浓度的升高导致AChE活性先增加后降低.综上,环境相关浓度下的IBP对海洋青鳉鱼胚胎及仔鱼不存在发育毒性,各浓度IBP均会导致胚胎产生氧化应激和神经毒性效应.To investigate the toxic effects of ibuprofen(IBP)on the early life stages of marine medaka(Oryzias melastigma),marine medaka embryos were exposed to different concentrations(0,1,10,100,and 1000μg·L^(-1))of IBP solution until 30 days after hatching(20 days for unhatched embryos).Various developmental parameters,including hatching rate,hatching time,heart rate,30-day survival rate of larvae,body length and weight,were observed and recorded.Additionally,measurements were conducted to assess the activity of antioxidant enzymes(superoxide dismutase,SOD;catalase,CAT;glutathione S-transferase,GST),levels of antioxidant(glutathione,GSH),concentrations of oxidative product(malonic dialdehyde,MDA),and the activity of neurotransmitter degrading enzyme(acetylcholinesterase,AChE)in the embryos.These measurements aimed to evaluate the developmental toxicity,antioxidant system,and neurotransmitter system effects of Ibuprofen on the early life stages of marine medaka.The results indicated that IBP at a concentration of 1μg·L^(-1) did not induce developmental toxicity in marine medaka embryos and larvae.However,exposure to higher concentrations resulted in decreased hatching rate,hatching time,heart rate,and larval survival rate,while no significant changes in body length and weight were observed across all concentrations.On the 5th day,increasing IBP concentration led to a decreasing trend in SOD activity and GSH content,while CAT and GST activities,as well as MDA levels,initially increased and then decreased,with all indicators returning to normal levels by the 8th day.Moreover,the increase in IBP concentration led to an initial increase and subsequent decrease in AChE activity.In conclusion,environmentally relevant concentrations of IBP did not exhibit developmental toxicity in marine medaka embryos and larvae.However,all tested concentrations of IBP resulted in oxidative stress and neurotoxic effects in the embryos.

关 键 词：布洛芬 海洋青鳉鱼 发育毒性 抗氧化系统 神经递质系统 

分 类 号：X-1[环境科学与工程] O6[理学—化学]