蝙蝠蛾拟青霉Cs-4菌粉有效成分的抗肿瘤作用  

Anti-Tumor Function of Active Ingredients from Paecilomyces hepiali CS-4

作　　者：闫芳 毕建丰 刘楚一 包海鹰[1] 杨明 YAN Fang;BI Jianfeng;LIU Chuyi;BAO Haiying;YANG Ming(Key Laboratory of Medicine Fungus Resources,Development and Utilization,Jilin Agriculture University,Changchun 130118,China;Jiangxi Guoyao Ltd.,Nanchang 330052,China)

机构地区：[1]吉林农业大学药用菌物资源及开发利用重点研究室,长春130118 [2]江西国药有限责任公司,南昌330052

出　　处：《菌物研究》2024年第3期257-267,共11页Journal of Fungal Research

基　　金：国家中药标准化项目(ZYBZH-C-JX-39)。

摘　　要：为评估蝙蝠蛾拟青霉Cs-4菌粉中5种有效成分联合给药的抗肿瘤活性,并探究其机理,采用小鼠腹水H22肝癌荷胞建立小鼠模型,将小鼠随机分成空白组、模型组、阳性组(环磷酰胺)、多糖组、腺苷组、几丁质组、麦角甾醇组、过氧麦角甾醇组和5种成分联合给药组,每组10只,连续给药10 d后测量剥离肿瘤组织并称重,计算抑瘤率和脏器指数,检测小鼠血清中IL-6、IL-2、TNF-α、IFN-γ、VEGF、AST和BUN细胞因子水平,肿瘤组织中VEGF、P53、Bcl-2、Bax和Caspase-3抗体的免疫反应,Caspase-3、Bax、Bcl-2和VEGF蛋白表达水平以及TUNEL法检测细胞凋亡情况。结果表明,蝙蝠蛾拟青霉Cs-4菌粉的5种有效成分联合给药可以通过调节多种细胞免疫因子水平达到多通路抗肿瘤效果。The present study was conducted to understand the anti-tumor activity and pharmacological mechanisms of effective ingredients extracted from Paecilomyces hepiali Cs-4.By using the mouse H22 cell line of abdominal hepatoma ascites,experimental samples were randomly divided into various groups,each containing ten mice,with or without treatments of five components;polysaccharide,adenosine,chitin,ergosterol and ergosterol peroxide.After ten days of continuous feeding,tumor tissues were taken out and body-mass detection was carried out for the calculating of tumor growth rate and organ index.Cytokine levels of IL-6,IL-2,TNF-α,IFN-γ,VEGF,AST and BUN in the mouse serum,immune responses of VEGF,P53,Bcl-2,Bax and Caspase-3 antibody in the tumor tissues and expression levels of Caspase-3,Bax,Bcl-2 and VEGF proteins were investigated.Status of cell death(apoptosis)was also measured via the TUNEL assay.Our results showed that the combined feeding of five active ingredient components of P.hepiali Cs-4 could generate a positive and multi-way effect on the anti-tumor process through regulation of immune factors in various cells.

关 键 词：抑瘤率 病理切片染色 细胞因子 细胞凋亡 蛋白免疫印迹 

分 类 号：Q949.32[生物学—植物学] R285.5[医药卫生—中药学]