灵芝子实体提取物对C57BL/6小鼠黑色素瘤肺转移的抑制作用  

Inhibitory Effect of Ganoderma Fruiting Body Extracts on Lung Metastasis of Melanoma in C57BL/6 Mice

作　　者：王芷宁 朱奕囡 谢文琦 张林波[1,2,3] WANG Zhining;ZHU Yinan;XIE Wenqi;ZHANG Linbo(College of Life Science,Jilin Agricultural University,Changchun 130118,China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Changchun 130118,China;College of Food Science and Pharmaceutical Engineering,Zaozhuang University,Zaozhuang 277160,China)

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]食药用菌教育部工程研究中心,长春130118 [3]枣庄学院食品科学与制药工程学院,枣庄277160

出　　处：《菌物研究》2024年第3期276-282,共7页Journal of Fungal Research

基　　金：贵州省食用菌育种重点实验室开放课题项目(黔科合平台人才[2019]5105-2008号)。

摘　　要：为进一步探究灵芝抗肿瘤活性及相关作用机制,建立C57BL/6小鼠黑色素瘤肺转移模型,从灵芝子实体中分别提取灵芝醇提物及灵芝水溶性多糖,并以50,100,200 mg/kg剂量对小鼠模型进行药物干预。给药2周后取小鼠肺脏,计算各组小鼠肺质量、肺指数、转移灶数目及抑瘤率,采用HE染色法观察肺组织病理变化,采用RT-qPCR法检测肺组织中与细胞外基质降解和细胞凋亡相关基因的mRNA表达变化,探讨灵芝醇提物及灵芝水溶性多糖对小鼠黑色素瘤肺转移的影响及可能机制作用。结果表明:灵芝醇提物和灵芝水溶性多糖均可降低小鼠的肺质量及肺指数,减少其转移灶数目;灵芝醇提物通过上调MMP-2、MMP-9表达、下调TIMP-2的表达抑制小鼠黑色素瘤肺转移的发生,而灵芝多糖可通过上调Bcl-2、下调Bax和Caspase-3的表达抑制小鼠黑色素瘤肺转移。该结果为灵芝活性研究及相关产品开发提供了实验依据。In order to further explore the antitumor activity and its functional mechanism of Ganoderma,a C57BL/6 mouse model of melanoma lung metastasis was established.Drug treatment respectively with the ethanol extracts(EEGL)and polysaccharides(GL-PS)derived from Ganoderma fruiting bodies was performed to the mouse model at doses of 50,100 and 200 mg/kg.After two weeks,lungs of mice in each group were taken out for morphological analysis.Investigation of the pathological changes through HE staining and expression activity of genes related to extracellular matrix degradation and apoptosis in lung tissues through RT-qPCR were also carried out to understand the effects and possible mechanisms of EEGL and GL-PS on metastasis of melanoma in mouse lungs.Our results showed that both of the supply of EEGL and GL-PS could reduce the lung weight and lung index,and the number of metastases in mice as well.The EEGL was found to inhibit the metastasis of melanoma in mouse lungs by up-regulating the gene expression of MMP-2 and MMP-9,and down-regulating the gene expression of TIMP-2.The GL-PS could inhibit the lung metastasis of mouse melanoma,on the other side,by up-regulating the gene expression of Bcl-2 and down-regulating the gene expression of Bax and Caspase-3.The present study thus provides available experiment data for our future research on the bioactivity of Ganoderma and therefore the development of its related products.

关 键 词：灵芝 肿瘤 肺转移 黑色素瘤 凋亡 

分 类 号：R967[医药卫生—药理学]