水母毒素磷脂酶A2在水母蜇伤致炎中的作用分析  

Analysis of the role of jellyfish toxin phospholipase A2 in inflammation induced by jellyfish sting

作　　者：王增发 陈镜伯 杨凤玲 王艺 刘珂彤 张晶[2] 肖良 WANG Zengfa;CHEN Jingbo;YANG Fengling;WANG Yi;LIU Ketong;ZHANG Jing;XIAO Liang(Naval Environment and Labor Hygiene Teaching and Research Office,Faculty of Naval Medicine,Naval Medical University,Shanghai,200433,China;College of Chinese Medicine,Jilin Agricultural University)

机构地区：[1]海军军医大学海军医学系海军环境与劳动卫生教研室,上海200433 [2]吉林农业大学中药材学院

出　　处：《临床急诊杂志》2024年第8期413-419,共7页Journal of Clinical Emergency

基　　金：国家重点研发计划资助(No:2022-3.3)。

摘　　要：目的:探究水母磷脂酶A2结构关系在水母蜇伤导致的全身炎症反应综合征中的作用。方法:基于沙海蜇、海月水母、安朵先水母、海蜇水母触手组织的转录组和蛋白组数据,利用生物信息学的分析手段对其理化性质、亲水性、信号肽、二级结构以及三级结构进行分析预测。结果:所得到的各类水母磷脂酶A2氨基酸序列,其蛋白主要以低分子量为特征,亲水性分析大部分蛋白都具有极好的亲水性特征,同时蛋白质二级结构和三级结构显示这些序列扭曲和折叠较多,丰富的结构特征对其生物活性发挥了重要作用。而通过STRING数据库构建蛋白互作网络发现磷脂酶A2序列都与PLA2G15、磷脂酶A2组Ⅲ(PLA2G3)、内收素1(ADD1)等蛋白质互相作用,该类蛋白质在各种炎症介质的产生中发挥了重要作用。结论:水母磷脂酶A2在水母蜇伤后导致的全身炎症反应综合征中可能是通过花生四烯酸等途径诱导了炎症反应,为水母蜇伤的治疗提供参考价值。Objective:To investigate the role of jellyfish phospholipase A2(PLA2)structural relationships in the systemic inflammatory response syndrome caused by jellyfish stings.Methods:Based on the transcriptomic and proteomic data of various jellyfish tentacle tissues,the physicochemical properties,hydrophilicity,signal peptide,secondary structure,and tertiary structure were analyzed and predicted via bioinformatics analysis.Results:The obtained amino acid sequences of various jellyfish PLA2,their proteins are mainly characterised by low molecular weight,and most of the proteins in the hydrophilicity analysis have excellent hydrophilicity characteristics,considering that the protein secondary and tertiary structures exhibit significant distortion and folding.Those rich structural features play an important role in their biological activities.And STRING database-based bioinformatics analysis found that those sequences all interact with PLA2G15,PLA2G3,ADD1 and other proteins by constructing protein interaction network,and this type of proteins plays an important role in the production of various inflammatory mediators.Conclusion:Jellyfish PLA2 may have induced the inflammatory response in the systemic inflammatory response syndrome caused by jellyfish stings through pathways such as Arachidonic acid metabolism,providing reference value for the treatment of jellyfish stings.

关 键 词：水母 磷脂酶A2 生物信息学 

分 类 号：R282.77[医药卫生—中药学]