瑞香狼毒对佐剂性关节炎大鼠相关细胞因子及肠道菌群的影响  

Effects of Stellera chamaejasme L.on related cytokines and intestinal flora in adjuvant arthritis rats

作　　者：胡伊力格其[1,2] 白小红 白梅荣 包文双[1] 胡和珠拉 敖日格乐 Huyiligeqi;BAI Xiaohong;BAI Meirong;BAO Wenshuang;Huhezhula;Aorigele(Mongolian Medical College,Inner Mongolia Minzu University,Tongliao 028000,China;Key Laboratory of Mongolian Medicine R&D Engineering,Ministry of Education,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学蒙医药学院,通辽028000 [2]蒙医药研发工程教育部重点实验室,通辽028000

出　　处：《中华中医药杂志》2024年第9期4953-4958,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：内蒙古自治区自然科学基金项目(No.2018LH08063,No.2020LH08013);内蒙古民族大学博士科研启动基金项目(No.B5408);内蒙古民族大学中药学(蒙药学)重点学科建设项目(No.ZYX005);内蒙古自治区高等学校项目(No.NMGIRT2216)。

摘　　要：目的:观察瑞香狼毒(SCL)对佐剂性关节炎(AA)大鼠相关细胞因子及肠道菌群的影响。方法:雄性SD大鼠于第1、7、14天右足后跖皮下注射弗氏完全佐剂0.1 mL建立AA模型,第8天开始阳性组灌胃雷公藤多苷片12.86 mg/kg,SCL高、中、低剂量组分别灌胃SCL 32.97、16.49、8.24 g/kg,共给药21 d,通过统计大鼠足跖肿胀度、组织病理学评价SCL对AA的保护作用。基于16S rRNA测序,ELISA法探讨SCL对AA大鼠肠道菌群及炎症因子的影响。结果:SCL可明显改善AA大鼠的关节病理症状,大鼠SCL给药组足跖肿胀度显著降低。与模型组比较,SCL高剂量组血清中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、IL-1β、雌二醇(E2)、类风湿因子(RF)水平显著降低(P<0.01,P<0.05),孕酮(PROG)水平显著升高(P<0.01)。SCL中剂量组血清中TNF-α、IL-6、RF水平显著降低(P<0.01,P<0.05),E2水平显著升高(P<0.01)。SCL低剂量组血清中TNF-α水平显著降低(P<0.05)。阳性组血清中TNF-α、IL-6、PROG水平显著降低(P<0.01)。在肠道菌群多样性方面,SCL显著升高了拟杆菌门、拟杆菌属、S24-7科、普雷沃氏菌科、拟杆菌目、S24-7纲、普雷沃氏菌纲、肠球菌纲、乳杆菌纲相对丰度(P<0.05),降低了瘤胃球菌科、梭菌目、乳杆菌目、颤螺菌纲相对丰度(P<0.05)。结论:SCL可能通过调节肠道菌群失衡状态发挥抗炎作用,进而治疗AA,为AA临床上的新药研发提供理论依据。Objective:To observe the effects of Stellera chamaejasme L.(SCL)on related cytokines and intestinal flora in adjuvant arthritis(AA)rats.Methods:The AA model was established by subcutaneous injection of O.1 mL Freund's complete adjuvant into the right hind paw of male SD rats on the Ist,7th and 14th days.From the 8 th day,the positive group was given 12.86 mg/kg tripterygium wilfordii polyglycoside tablets by gavage,and the high,medium and low dose groups of SCL were given 32.97,16.49 and 8.24 g/kg respectively by gavage for 21 days.The protective effect of SCL on AA was evaluated by statistics of rat paw swelling and histopathology.Based on 16S rRNA sequencing,the effects of SCL on intestinal flora and inflammatory factors in AA rats were investigated by ELISA.Results:SCL could significantly improve the joint pathological symptoms of AA rats,and the paw swelling degree of rats in SCL administration group was significantly reduced.Compared with the model group,the levels of serum tumor necrosis factor-α(TNF-α),interleukin(IL)-6,IL-1β,estradiol(E2)and rheumatoid factor(RF)in the high-dose SCL group were significantly decreased(P<0.01,P<0.05),and the level of progesterone(PROG)was significantly increased(P<0.01).The levels of TNF-α,IL-6 and RF in serum of SCL medium dose group were significantly decreased(P<0.01,P<0.05),and the level of E,was significantly increased(P<0.01).The level of TNF-αin serum of SCL low dose group was significantly decreased(P<0.05).The serum levels of TNF-α,IL-6 and PROG in the positive group were significantly decreased(P<0.01).In terms of intestinal flora diversity,SCL significantly increased the relative abundance of bacteroidetes,bacteroides,S247 family,prevotaceae,bacteroidales,S247class,prevotclass,enterococcus class,lactobacillus class(P<0.05),and decreased the relative abundance of coccidiaceae,alostridium,lactobacillaceae,and frium(P<0.05).Conclusion:SCL may play an antiinflammatory role by regulating the imbalance of intestinal flora,and then treat AA,and provides a theoreti

关 键 词：瑞香狼毒 佐剂性关节炎 细胞因子 肠道菌群 16S rRNA 

分 类 号：R285.5[医药卫生—中药学]