二代测序在假体周围感染诊断应用的进展  

Progress of second generation sequencing in the diagnosis of periprosthetic joint infection

作　　者：刘永飞 张杰 曹建泽 赵海燕 LIU Yong-fei;ZHANG Jie;CAO Jian-ze;ZHAO Hai-yan(The First Clinical College,Lanzhou University,Lanzhou 730000,China;Department of Orthopaedics,First Hospital,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学第一临床医学院,甘肃兰州730000 [2]兰州大学第一医院骨科,甘肃兰州730000

出　　处：《中国矫形外科杂志》2024年第17期1576-1581,共6页Orthopedic Journal of China

基　　金：国家自然科学基金项目(编号:82060394);兰州市人才创新创业项目(编号:2020-RC-45);兰州大学第一医院院内基金项目(编号:ldyyyn2022-73)。

摘　　要：假体周围感染是关节置换术后的一种严重并发症,需要大量的医疗资源,给患者和社会带来了巨大的经济负担。近年来,随着新型分子诊断技术的不断发展,假体周围感染的诊断和治疗取得了重大进展。与传统培养相比,二代测序可以快速、准确、全面地诊断致病微生物,提高假体周围感染中致病微生物的检出率,从而实现早期诊断和治疗假体周围感染。本文综述了二代测序的原理、方法和其在假体周围感染中识别致病微生物的特点、优势和局限性。Periprosthetic joint infection(PJI)is a serious complication after joint replacement,which requires a lot of medical resources and brings a huge economic burden to patients and society.In recent years,with the continuous development of novel molecular diagnostic techniques,the diagnosis and treatment of PJI have made great progress.Compared with traditional culture,the next-generation sequencing can diagnose pathogenic microorganisms quickly,accurately and comprehensively,and improve the detection rate of pathogenic microorganisms in PJI,so as to achieve early diagnosis and treatment of PJI.This paper reviews the principle and method of next-generation sequencing and its characteristics,advantages and limitations in identifying pathogenic microorganisms in PJI.

关 键 词：二代测序 假体周围感染 诊断 

分 类 号：R687[医药卫生—骨科学]