光交联纳米酶葡聚糖水凝胶的制备及应用  

Preparation and Application of Photo Cross-Linked Nanase Dextran Hydrogels

作　　者：庞洁 武翠翠 朱纤倩 张文柠 汤顺清[1] 李艳艳 PANG Jie;WU Cuicui;ZHU Qianqian;ZHANG Wenning;TANG Shunqing;LI Yanyan(College of Life Science and Technology,Jinan University,Guangzhou 510632,China)

机构地区：[1]暨南大学生命科学技术学院,广州510632

出　　处：《功能高分子学报》2024年第5期433-440,共8页Journal of Functional Polymers

基　　金：国家自然科学基金(32171312)。

摘　　要：通过酯交换反应,将甲基丙烯酸缩水甘油酯(GMA)接枝到葡聚糖(Dex)上,制备出具备光交联性能的甲基丙烯酰化葡聚糖(Dex-MA)。利用“一锅法”将姜黄素(Cur)负载在沸石咪唑酯骨架材料-8(ZIF-8)的框架内,合成具有pH响应性的沸石咪唑酯骨架-8@姜黄素(ZIF-8@Cur)。将ZIF-8@Cur引入Dex-MA溶液中,加入光引发剂I2959并通过紫外光照射引发其光交联,制备出具有良好力学性能和抗菌性能的光交联水凝胶(Dex-ZC)。通过核磁共振氢谱仪、紫外吸收光谱仪、傅里叶红外光谱仪、X射线衍射仪、扫描电子显微镜等对Dex-MA、ZIF-8@Cur和Dex-ZC水凝胶的结构和形貌进行分析,并通过拉伸实验和抑菌圈实验对Dex-ZC水凝胶的力学性能和抗菌性能进行探究。结果表明:ZIF-8@Cur在酸性环境下,释放速率更快,24 h内累积释放率达到(72.98±0.18)%;Dex-ZC水凝胶具有有序的疏松多孔结构和良好的力学性能。抑菌圈实验表明,加入带有正电荷的ZIF-8@Cur,使得Dex-ZC水凝胶对大肠杆菌和金黄色葡萄球菌具有优异的抗菌性能。The methacrylated dextran(Dex-MA)with photo cross-linked property was prepared by grafting glycidyl methacrylate(GMA)onto dextran(Dex)via an ester exchange reaction.Curcumin(Cur)was loaded in the framework of zeolitic imidazolate framework material(ZIF-8)by“one-pot”method,and then the pH-responsive ZIF-8@Cur was synthesized directly.The photo cross-linked hydrogels(Dex-ZC)with good mechanical and antibacterial properties were prepared by introducing ZIF-8@Cur nanase into Dex-MA solution,adding photo initiator I2959 and initiating photo crosslinking through ultraviolet irradiation.The structure and morphology of Dex-MA,ZIF-8@Cur and Dex-ZC were analyzed by nuclear magnetic resonance hydrogen spectrum,ultraviolet absorption spectrum,Fourier infrared spectroscopy,Xray diffraction and scanning electron microscope.The mechanical properties and antibacterial properties of Dex-ZC were studied by tensile test and bacteriostatic ring test.Results showed that the release rate of ZIF-8@Cur was faster in acidic environment,and the cumulative release amount of Cur reached(72.98±0.18)%within 24 h.Dex-ZC hydrogel had compact porous structure and good mechanical properties,which was caused by the addition of [e-mail] bacteriostatic ring test showed that the addition of positive charge ZIF-8@Cur made Dex-ZC hydrogel have excellent antibacterial activity against E.coli and S.aureus.This study provided more possibilities for the development of photo cross-linked hydrogel dressings on wound healing.

关 键 词：葡聚糖 甲基丙烯酸缩水甘油酯 金属有机框架 水凝胶 抗菌 

分 类 号：R318.08[医药卫生—生物医学工程]