2.5 T太赫兹辐射暴露对小鼠睾丸组织损伤效应及机制研究  

Effects and mechanisms of 2.5T terahertz radiation exposure on testis injury in mice

作　　者：马秦龙 林敏 高鹏[1] 杨玲玲 卢永辉[1] 张彦文[1] 余争平[1] 陈纯海[1] MA Qinlong;LIN Min;GAO Peng;YANG Lingling;LU Yonghui;ZHANG Yanwen;YU Zhengping;CHEN Chunhai(Department of Occupational Health,Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education,Faculty of Military Preventive Medicine,Army Medical University(Third Military Medical University),Chongqing,400038,China)

机构地区：[1]陆军军医大学(第三军医大学)军事预防医学系军队劳动卫生学教研室,电磁辐射医学防护教育部重点实验室,重庆400038

出　　处：《陆军军医大学学报》2024年第19期2197-2207,共11页Journal of Army Medical University

基　　金：国家自然科学基金NSAF联合基金(U2130107);重庆英才·优秀科学家项目(cstc2024ycjh-bgzxm0053)。

摘　　要：目的 探讨太赫兹(terahertz, THz)辐射暴露对小鼠睾丸组织的损伤效应及其潜在的分子机制。方法 采用125只6~8周龄雄性C57BL/6J小鼠,通过频点为2.5 T的THz辐射单次暴露动物,暴露平均功率密度为38、115和318 mW/cm^(2),暴露时间为5或10 min。于暴露结束后即刻或24 h分别采用HE染色检测病理损伤,采用ELISA检测睾丸组织炎症因子表达量,采用转录组测序检测睾丸组织基因表达变化整体情况,并通过生物信息学对差异表达基因进行聚类分析,筛选敏感基因并通过RT-qPCR检测其在THz辐射暴露后表达的时效量效关系,最后在显微镜下对精子质量进行形态学分析。结果 3个剂量THz辐射暴露均未造成小鼠睾丸组织显著病理损伤;平均功率密度115 mW/cm^(2)暴露后即刻TNF-α表达增高(P<0.01),THz剂量达到318 mW/cm^(2)后,IL-1β及TNF-α的表达均升高(P<0.01),但在24 h后均恢复至正常水平。转录组测序结果表明,THz辐射暴露能够造成56个基因表达异常,聚类分析结果表明,这些基因主要富集于免疫与炎症反应、酶活性、精子发育与获能等功能。对筛选出的关键基因Crisp1、Adam7、Ltf、Rnase9与Bsph1表达检测发现,115 mW/cm^(2)暴露后即刻Crisp1与Rnase9表达下调,剂量至318 mW/cm^(2)时,所有5个基因表达均发生显著变化(P<0.05),而24 h后均恢复至正常水平。对精子功能的形态学检测发现,3个剂量THz辐射暴露均不导致精子质量的变化。结论 THz辐射单次暴露引起睾丸组织的暂时性炎症反应和精子功能相关基因表达异常,但24 h后可恢复正常,不影响精子质量。Objective To investigate the effects of terahertz(THz)radiation on mouse testicular tissue and its potential molecular mechanisms.Methods A total of 125 male C57BL/6J mice(6~8 weeks old)were randomly divided into control group and low-,medium-and high-radiation power groups.The mice of the latter 3 groups were exposed to THz radiation at a frequency of 2.5 T,with an average power density of 38,115,or 318 mW/cm^(2),for 5 or 10 min.The detection time was immediately or 24 h after exposure.HE staining was used to observe pathological damage.ELISA was employed to detect the expression of inflammatory factors in testicular tissue.RNA-seq was utilized to detect the global changes of gene expression.The differentially expressed genes(DEGs)were screened and bioinformatics was used to cluster them.The screened genes were further analyzed with RT-qPCR to determine the time-dependent and dose-dependent relationships of the expression with THz exposure.Finally,sperm quality was evaluated morphologically using a microscope.Results Three doses of THz radiation exposure did not cause significant pathological damages to mouse testicular tissue.TNF-αexpression was increased immediately after exposure at average power density of 115 mW/cm^(2)(P<0.01),and the expression of IL-1βand TNF-αwere both increased when the dose reached 318 mW/cm^(2)(P<0.01).However,all the 3 factors returned to normal levels in 24 h after exposure.RNA-seq results showed that THz radiation exposure caused abnormal expression of 56 genes.Cluster analysis indicated that these DEGs were mainly enriched in immune and inflammatory responses,enzyme activity,sperm development and capacitation functions.Then for 5 selected key genes,Crisp1,Adam7,Ltf,Rnase9,and Bsph1,the expression of Crisp1 and Rnase9 was decreased immediately after exposure to 115 mW/cm^(2) THz radiation,the dose of 318 mW/cm^(2) resulted in obvious changes in the expression of the 5 genes(P<0.05),and their expression returned to normal levels in 24 h after exposure.Morphological observation di

关 键 词：太赫兹 睾丸 炎症反应 精子功能 转录组测序 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学] R322.64[医药卫生—基础医学] R818.023