蛇床苦参煎煮液抑制化合物48/80诱导ICR小鼠瘙痒的作用研究  

Shechuang-Kushen Decoction Inhibit Effect and Mechanism of Compound 48/80 on Itch in ICR Mice

作　　者：伍师坚 黄光羡 宋芊芊 黄春华 刘佳敏 伍冠一 WU Shijian;HUANG Guangxian;SONG Qianqian;HUANG Chunhua;LIU Jiamin;WU Guanyi(School of Basic Medicine,Guangxi University of Chinese Medicine,Nanning Guangxi 530299,China;Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine(Guangxi University of Chinese Medicine),Nanning Guangxi 530299,China;Key Laboratory of Characteristic Experimental Animal Models of Guangxi Universities(Guangxi University of Chinese Medicine),Nanning Guangxi 530299,China;School of Pharmacy,Guangxi University of Chinese Medicine,Nanning Guangxi 530200,China)

机构地区：[1]广西中医药大学基础医学院,广西南宁530299 [2]广西高发传染病中西医结合转化医学重点实验室(广西中医药大学),广西南宁530299 [3]广西特色实验动物模型重点实验室(广西中医药大学),广西南宁530299 [4]广西中医药大学药学院,广西南宁530200

出　　处：《广西师范大学学报（自然科学版）》2024年第5期163-173,共11页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(82060768);广西自然科学基金(2020GXNSFAA297244);广西研究生教育创新计划项目(YCSW2023392)。

摘　　要：蛇床子和苦参是常用止痒中药,但它们联合对瘙痒的作用尚未完全明确。本研究通过制备蛇床苦参煎煮液,灌胃ICR小鼠,采用行为学法观察药物对小鼠瘙痒行为的影响;伊文思蓝染色观察血管通透性变化;ELISA、WB法评价煎煮液对RBL-2H3细胞释放细胞因子的影响。结果发现:煎煮液抑制瘙痒行为以及血管通透性升高,但不影响小鼠探索能力、肌张力;煎煮液减少RBL-2H3细胞释放β-氨基己糖苷酶、组胺、IL-31、IL-4、IL-13和CXCL10,且能抑制ERK蛋白的磷酸化。本研究表明蛇床苦参煎煮液通过抑制RBL-2H3细胞脱颗粒和瘙痒相关细胞因子的释放来抑制化合物48/80诱导的急性瘙痒。Common Cnidium and Sophora flavescens are commonly used antipruritic herbs,but their combined effect on itch has not been fully elucidated.In this study,ICR mice were gavaged with Shechuang-Kushen Decoction,and the effect of the drug on the pruritus behavior of the mice was observed by a behavioral method.The changes in vascular permeability were observed by Evans Blue staining.The effect of the decoction on the release of cytokines from the mast cell line RBL-2H3 was evaluated by ELISA and Western Blotting,respectively.The results showed that the decoction inhibited pruritus behavior and increased vascular permeability,but did not affect the exploration ability or muscle tone of the mice.The decoction solution reduced the release ofβ-hexosaminidase,histamine,IL-31,IL-4,IL-13,and CXCL10,and inhibited the phosphorylation of the ERK protein in RBL-2H3 cells.This study demonstrated that Shechuang-Kushen Decoction inhibited compound 48/80-induced acute pruritus by inhibiting RBL-2H3 cell degranulation and the release of pruritus-related cytokines.

关 键 词：蛇床子 苦参 瘙痒 止痒 

分 类 号：R285.5[医药卫生—中药学]