东川泥石流源区板岩表生细菌生物多样性  

Biodiversity of Bacterial Communities Inhabiting Slate Surfaces of Debris Flow Areas in Dongchuan,China

作　　者：赵雯钰 杨宾 程诚 王琪 臧胜刚 盛下放[2] ZHAO Wenyu;YANG Bin;CHENG Cheng;WANG Qi;ZANG Shenggang;SHENG Xiafang(School of Applied Meteorology,Nanjing University of Information Science&Technology,Nanjing 210044,China;College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京信息工程大学应用气象学院,南京210044 [2]南京农业大学生命科学学院,南京210095

出　　处：《土壤》2024年第4期788-796,共9页Soils

基　　金：国家自然科学基金面上项目(41977040、42077288、42273079);高端外国专家引进计划项目(110000216220228007)资助。

摘　　要：选取云南省昆明市蒋家沟泥石流物源区低风化(LT组)和高风化(MT组)岩石样品以及流通区岩石(F组)样品为研究对象,利用细菌16Sr RNA基因序列高通量测序技术研究岩石表生细菌群落结构与多样性,并通过冗余分析(RDA)探究岩石样品理化性质对岩石表生细菌群落变异的驱动作用。结果表明:该区域岩石属于板岩;LT组岩石样品细菌群落α多样性指数(包括Faith’s系统发育多样性指数(PD)、Shannon指数、Chao1指数和每个样品具有的独特OTU数目)均显著高于MT组和F组岩石样品,且α多样性指数与板岩有机质含量呈显著正相关(r=0.80~0.91,P<0.01)。该区域板岩表生细菌的优势种群包括Firmicutes(厚壁菌门,相对丰度66%)、Proteobacteria(变形菌门,15%)和Actinobacteria(放线菌门,11%)。PCoA和UPMGA聚类分析结果显示3组岩石样品的表生细菌群落存在显著差异。Firmicutes菌门的相对丰度由高到低分别为MT组>F组>LT组,Actinobacteria和Proteobacteria菌门的相对丰度由高到低分别为LT组>F组>MT组。RDA结果显示板岩理化性质解释了该区域岩石样品93%的群落变异,其中有效态Ca含量是板岩表生细菌群落结构变异的最主要驱动因素,解释了36.6%的群落结构变异。相关性结果表明优势菌门(除Firmicutes外)的相对丰度均与有效态Si和Mn含量呈显著正相关,而与有效态Ca含量呈显著负相关。本研究进一步丰富了岩石表生微生物物种资源及表生微生物群落变化的驱动机制。The low weathered(LT)and high weathered(MT)rock samples from the source area,and the rock samples from the circulation area(F)of Jiangjiagou debris flow in Kunming City,Yunnan Province were collected as the materials.The high-throughput sequencing of bacterial 16S rRNA gene sequence was used to study the community structure and diversity of bacterial communities inhabiting rock surface,and the driving effects of the physiochemical properties of rock samples on the variation of rock epiphytic bacteria community were explored through redundancy analysis(RDA).The results indicated that the rocks in this area belonged to slate.Theα-diversity indexes of bacterial community in rock samples of LT group(including Faith's phylogenetic Diversity index(PD),Shannon index,Chao1 index and the number of unique OTUs in each sample)were significantly higher than those in rock samples of MT group and F group,andα-diversity index was significantly positively correlated with the organic matter content of slate(r=0.80–0.91,P<0.01).The dominant populations of surface bacteria in the slate included Firmicutes(with relative abundance of 66%),Proteobacteria(15%)and Actinobacteria(11%).The results of PCoA and UPMGA showed significant differences in the bacterial communities of the three rock samples.The relative abundance of Firmicutes from high to low was MT group>F group>LT group,while the relative abundances of Actinobacteria and Proteobacteria from high to low were LT group>F group>MT group.RDA results showed that physiochemical properties of slate explained 93%of the variation of rock sample community,and the content of available Ca was the main driver for the variation of the bacterial community inhabiting salte surface,which explained 36.6%of the toral variation.The relative abundance of dominant phyla(except for Firmicutes)were significantly positively correlated with the contents of available Si and Mn,while negatively correlated with the content of available Ca.This study further enriches the resources of rock surface microb

关 键 词：板岩 高通量测序 表生细菌 生物多样性 群落结构 

分 类 号：Q93[生物学—微生物学]