烟草NPR1基因家族鉴定及其在南方根结线虫胁迫下的表达分析  

Identification of Tobacco NPR1 Gene Family and Expression Analysis Under Meloidogyne incognita Stress

作　　者：刘勇 杨志晓 孔德钧 王仁刚 黄宁 余世洲 王世泽 张小全[1] Liu Yong;Yang Zhixiao;Kong Dejun;Wang Rengang;Huang Ning;Yu Shizhou;Wang Shize;Zhang Xiaoquan(College of Tobacco,Henan Agricultural University,Zhengzhou 450002,China;Guizhou Academy of Tobacco Science,Guiyang 550081,China;Guiyang Branch of Guizhou Tobacco Company,Guiyang 550002,China;College of Agriculture and Biotechnology,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China)

机构地区：[1]河南农业大学烟草学院,河南郑州450002 [2]贵州省烟草科学研究院,贵州贵阳550081 [3]贵州省烟草公司贵阳市公司,贵州贵阳550002 [4]河北科技师范学院农学与生物科技学院,河北秦皇岛066004

出　　处：《山东农业科学》2024年第9期22-33,共12页Shandong Agricultural Sciences

基　　金：中国烟草总公司科技重大专项项目[11020200027(JY-10)];贵州省烟草公司科技项目(2022XM05);遵义市烟草公司科技项目(2021XM05)。

摘　　要：本研究旨在挖掘烟草抗南方根结线虫病程相关基因非表达子NPR1(non-expressor of PR genes)家族成员,并明确其功能。基于拟南芥AtNPR1家族成员蛋白序列,利用生物信息学方法鉴定分析烟草NtNPR1基因家族成员及其特征。通过qRT-PCR检测南方根结线虫侵染后抗病(NC95)、感病(长脖黄)烟草品种Nt-NPR1家族重要候选抗性基因的相对表达量,采用LC-MS/MS方法对2个品种烟草根系内源水杨酸含量进行检测。结果表明,从普通烟草基因组中共鉴定到12个NtNPR1基因,这些基因被分为3个亚组,同一亚组中NtNPR1家族成员蛋白结构、保守基序的长度和位置都极为相似。编码氨基酸长度范围为462~588,均为亲水性蛋白,均位于细胞核。南方根结线虫侵染抗、感烟草品种后,根系中水杨酸含量、NtNPR1基因家族成员表达量在抗病品种中均升高,在感病品种中则降低,其中NtNPR6的变化最为明显。综上,NPR1基因与烟草南方根结线虫抗性密切相关,NtNPR6基因可作为烟草响应南方根结线虫胁迫的重要基因。The research was aimed to mining the non-expressor of PR genes(NPR1)family members in-volved in tobacco resistance to the Meloidogyne incognita disease.Members of the tobacco NtNPR1 gene family were identified and analyzed using bioinformatics method based on the protein sequences of AtNPR1 family members in Arabidopsis.Relative expression level of key candidate resistance genes of NtNPR1 family in tobac-co resistant(NC95)and susceptible(Changbohuang,CBH)varieties infected by M.incognita were analyzed by qRT-PCR.The endogenous salicylic acid(SA)content in roots of the two tobacco varieties were measured by LC-MS/MS.The results showed that a total of 12 NtNPR1 genes were identified from the genome of com-mon tobacco,which were classified into three subgroups.Within each subgroup,the protein structure and con-served motif length and position of NtNPR1 family members were highly similar.The length of encoded amino acids ranged between 462 and 588 aa,and all of them were hydrophilic and localized in nucleus.After being infected with M.incognita,the SA content and expression level of NtNPR1 family members in roots increased in the resistant variety and decreased in the susceptible variety,among which,NtNPR6 changed the most ob-viously.In conclusion,the NPR1 gene is closely related to tobacco resistance to M.incognita,and the Nt-NPR6 gene could be used as a critical gene in response to M.incognita stress.

关 键 词：烟草 NPR1基因家族 生物信息学分析 南方根结线虫 表达分析 

分 类 号：Q781[生物学—分子生物学] S572[农业科学—烟草工业]