电针介导CXCR7/PI3K/Akt通路改善糖尿病慢性炎性脱髓鞘性多发性神经根神经病小鼠神经炎症  

Electroacupuncture improve neuroinflammation in mice with diabetic chronic inflammatory demyelinating polyradiculoneuropathy by mediating CXCR7/PI3K/Akt pathway

作　　者：刘洋洋 董春蕊 孙新瑶 李春曼 LIU Yang-yang;DONG Chun-rui;SUN Xin-yao;LI Chun-man(Section 7,Department of Neurology,Cangzhou People's Hospital,Cangzhou 061002,China)

机构地区：[1]沧州市人民医院神经内科七区,河北沧州061002

出　　处：《解剖科学进展》2024年第3期240-243,246,共5页Progress of Anatomical Sciences

基　　金：沧州市重点研发计划(213106095)。

摘　　要：目的基于CXCR7/PI3K/Akt通路探讨电针调节巨噬细胞极化改善糖尿病慢性炎性脱髓鞘性多发性神经根神经病小鼠神经炎症的作用机制。方法将40只敲除共刺激蛋白CD86非肥胖糖尿病背景的小鼠随机分为模型组、电针DCIDP、电针+AMD3100组及电针+AMD3100+LY294002组,另取同龄C57BL/6小鼠作为对照组。检测各组小鼠神经评分;HE染色观察各组小鼠坐骨神经病理损伤和神经元损伤情况;ELISA检测各组小鼠坐骨神经TNF-α、IFN-γ及IL-4表达水平;免疫荧光染色检测各组小鼠M1型巨噬细胞标志蛋白(iNOS)/CD68、M2型巨噬细胞标志蛋白(Arg-1)/CD68的表达水平;Western blot检测各组小鼠坐骨神经CXCR7、PI3K、Akt、p-PI3K及p-Akt表达。结果与模型组相比,电针组、电针+AMD3100组CXCR7、p-PI3K、p-Akt蛋白表达显著升高;神经评分降低,坐骨神经病理损伤减轻,TNF-α、IFN-γ表达水平降低、IL-4表达水平升高、iNOS/CD68阳性表达水平显著降低、Arg-1/CD68阳性表达水平显著升高;然而电针、AMD3100对小鼠的上述作用均被LY294002削弱或抑制。结论电针可能是通过调控CXCR7/PI3K/Akt通路活性,促进M1型巨噬细胞向M2型巨噬细胞极化,进而改善糖尿病慢性炎性脱髓鞘性多发性神经根神经病小鼠神经炎症。Objective To explore the mechanism of electroacupuncture in regulating macrophage polarization and improving neuroinflammation in mice with diabetic chronic inflammatory demyelinating polyradiculoneuropathy based on CXCR7/PI3K/Akt pathway.Methods Mice with a non-obese diabetic background knocked out of the co-stimulator protein CD86 were randomly divided into model group,electroacupuncture group,electroacupuncture+AMD3100 group and electroacupuncture+AMD3100+LY294002 group,and C57BL/6 mice of the same age were used as the control group.Neurological function scores were detected.The sciatic neuropathic injury was observed by HE staining.The levels of TNF-α,IFN-γand IL-4 were measured by ELISA.The expressions of M1 macrophage marker protein(iNOS)and M2 macrophage marker protein(Arg-1)were detected by immunofluorescence staining.The expressions of CXCR7,PI3K,Akt,p-PI3K and p-Akt proteins were detected by Western blot.Results Compared with model group,the neurological function score was significantly decreased in electroacupuncture and electroacupuncture+AMD3100 group,the sciatic neuropathological injury was decreased,the levels of TNF-αand IFN-γwere significantly decreased,the level of IL-4 was significantly increased,the level of iNOS was significantly decreased,and the expression of Arg-1 was significantly increased.The expressions of CXCR7,p-PI3K and p-Akt were significantly increased.However,the above effects of electroacupuncture and AMD3100 on mice with mental disorders were weakened or inhibited by LY294002.Conclusion Electroacupuncture may promote the polarization of M1 macrophages to M2 by regulating the activity of CXCR7/PI3K/Akt pathway,thus improving the neuroinflammation of diabetic chronic inflammatory demyelinating polyradiculopathy mice.

关 键 词：糖尿病慢性炎性脱髓鞘性多发性神经根神经病 神经炎症 电针 巨噬细胞 极化 CXCR7/PI3K/Akt通路 

分 类 号：R587[医药卫生—内分泌]