The relationship between the distribution of HBcAg in liver cells and the pathological manifestations of the liver in 429 HBeAg-positive chronic HBV-infected individuals  

作　　者：Fan Nie Chang-Chun Zeng Guang-Dong Tong Mu-Min Shao Xiao-Hua Le Xin Deng Da-Qiao Zhou 

机构地区：[1]National Clinical Research Center for Infectious Diseases,Shenzhen 518112,China [2]Central Laboratory of Shenzhen Longhua District Central Hospital,Shenzhen 518112,China [3]Hepatology Department of Shenzhen Traditional Chinese Medicine Hospital,Shenzhen 518112,China [4]Pathology Department of Shenzhen Traditional Chinese Medicine Hospital,Shenzhen 518112,China [5]Pathology Department of Shenzhen Third People's Hospital,Shenzhen 518112,China [6]Traditional Chinese Medicine Department of Shenzhen Third People's Hospital,Shenzhen 518112,China

出　　处：《Gastroenterology & Hepatology Research》2024年第3期10-15,共6页胃肠与肝病学杂志（英文）

摘　　要：Background:The natural history of chronic HBV infection is typically characterized by four stages:the immune tolerance period,the immune clearance period,the immune control period,and the immune escape period.These stages are associated with the distribution of HBcAg in liver cells;however,this relationship remains a topic of broad debate within the field of liver disease.To objectively and quantitatively measure the intracellular distribution of HBcAg,this paper aims to design a method referred to as the“layered evaluation method”and to examine its validation.Methods:The distribution of HBcAg in liver cells is assessed using Image Pro Plus image processing software,along with calculations of cytoplasmic and nuclear positive staining rates.Results:The findings indicate that the highest proportion of patients exhibited a positive cytoplasmic expression rate ranging from 0-2.5%.More than 40% of the total sample was categorized within the 0-2.5% positive nuclear expression range.The HBcAg cytoplasmic positive staining rates were classified into five levels:a cytoplasmic HBcAg positive staining rate of less than 0.05% is designated as level 0,indicating negative expression;a staining rate between 0.05% and 5% is classified as level 1;a rate from 5% to less than 10% is classified as level 2;a rate from 10% to less than 20% is classified as level 3;and a nuclear positivity rate exceeding 20% is classified as level 4.Conclusion:The inflammatory activity grade in these patients was positively correlated with the cytoplasmic distribution of HBcAg.Furthermore,the nuclear distribution rate of HBcAg was significantly higher in the G3 group compared to the other groups.

关 键 词：distribution of HBcAg pathological manifestations of the liver positive staining rate 

分 类 号：R51[医药卫生—内科学]