基于matK、psbA-trnH、racL-a、trnL-trnF序列分析枸杞古树的遗传多样性  

Genetic Diversity of Ancient Lycium was Analyzed Based on matK,psbA-trnH,racL-a and trnL-trnF Sequences

作　　者：胡永超 马洁 朱金忠 杨涓[1] 郑蕊[1] 张磊[2] 郑国琦[1] Hu Yongchao;Ma Jie;Zhu Jinzhong;Yang Juan;Zheng Rui;Zhang Lei;Zheng Guoqi(College of Life Science,Ningxia University,Yinchuan,750021;School of Bioscience and Engineering,Northern University for Nationalities,Yinchuan,750021;Ningxia Zhongning County Qixin Seed Industry Co.,Ltd.,Zhongning,755100)

机构地区：[1]宁夏大学生命科学学院,银川750021 [2]北方民族大学生物科学与工程学院,银川750021 [3]宁夏中宁县杞鑫枸杞种业有限公司,中宁755100

出　　处：《分子植物育种》2024年第19期6375-6381,共7页Molecular Plant Breeding

基　　金：宁夏自然科学基金项目(2021AAC02005);宁夏大学研究生创新项目(GIP2020048);宁夏枸杞产业发展中心项目共同资助。

摘　　要：枸杞作为宁夏地区重要的药用植物之一,其营养价值、经济价值和生态价值对于宁夏地区都有着重要的意义。枸杞古树资源是枸杞育种重要的种质资源之一。本研究基于叶绿体基因matK、psbA-trnH、racL-a、trnL-trnF序列分析枸杞古树的遗传多样性。研究结果表明,四段基因全长2892 bp,保守位点为2863 bp,变异位点为29 bp,变异率达0.01%。以黑果枸杞(L37)为外类群,构建系统发育树,结果显示所有古树遗传距离较小,表明样品古树遗传年限较短,尚未形成独立群体。本研究通过四段叶绿体基因分析枸杞古树的遗传多样性,为后续枸杞引种驯化和育种提供了理论依据,对于后续枸杞生产实践有着重要意义。Lycium is one of the important medicinal plants in Ningxia,and its nutritional value,economic value and ecological value are of great significance to Ningxia.Lycium ancient tree resources is one of the important germplasm resources in Lycium breeding.In this study,the genetic diversity of ancient Lycium barbarum was analyzed based on the sequences of chloroplast genes matK,psbA-trnH,racL-a and trnL-trnF.The results showed that the full length of the four genes was 2892 bp,the conservative site was 2863 bp,the mutation site was 29 bp,and the mutation rate was 0.01%.Taking Lycium L37 as an outgroup,a phylogenetic tree was constructed.The results showed that the genetic distance of all the ancient trees was small,indicating that the genetic years of the sample ancient trees were short and had not yet formed an independent population.In this study,four-segment chloroplast genes were used to analyze the genetic diversity of ancient Lycium trees,which provided a theoretical basis for subsequent introduction,domestication and breeding of Lycium,and was of great significance for the production practice of Lycium.

关 键 词：枸杞古树 叶绿体基因 遗传多样性 

分 类 号：S567.19[农业科学—中草药栽培]