金银花及近缘种遗传多样性分析及SSR指纹图谱构建  

Genetic Diversity Analysis and SSR Fingerprint Construction of Lonicera japonica and Its Related Species

作　　者：侯立娜 李慧[1,2] 王天琪 徐瑞瑞 付晓 李圣波 刘忠华[1,2] Hou Lina;Li Hui;Wang Tianqi;Xu Ruirui;Fu Xiao;Li Shengbo;Liu Zhonghua(National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration,Beijing Forestry University,Beijing,100083;College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing,100083;Pingyi County Honeysuckle Nectar Development Promotion Center,Pingyi,273300;Shandong Yate Eco-Tech Co.,Ltd.,Linyi,266071)

机构地区：[1]北京林业大学,林木遗传育种国家工程实验室,林木花卉遗传育种教育部重点实验室,国家林业局树木花卉育种与生物工程重点开放实验室,北京100083 [2]北京林业大学生物科学与技术学院,北京100083 [3]平邑县金银花果茶发展促进中心,平邑273300 [4]山东亚特生态技术股份有限公司,临沂266071

出　　处：《分子植物育种》2024年第19期6382-6389,共8页Molecular Plant Breeding

基　　金：国家林业和草原局业务委托项目(2020104020);北京市园林绿化局计划项目(2021-STBHXFC-04-11)共同资助。

摘　　要：为了解金银花及近缘种之间的遗传背景差异与亲缘关系远近,本研究利用SSR分子标记技术对35份药用金银花种质资源进行遗传多样性分析和指纹图谱构建。结果表明,所选用的23对引物有效等位基因数和期望杂合度的平均值分别为2.891和0.618,Shannon信息指数和Nei's遗传多样性指数的平均值分别为1.300和0.609,各位点的多态性信息含量平均值为0.597,表现出较高的多态性。在遗传相似系数为0.250处,可将35份供试材料分为4类。本研究运用核心引物法构建了金银花及其近缘种的指纹图谱,实现了从分子水平上对金银花种质资源的快速鉴别。本研究为区分金银花真伪品、丰富优良性状和选育优质品种提供了科学依据。In order to understand the differences of genetic background and genetic relationship between Lonicera japonica germplasm resources,in this study,genetic diversity analysis and fingerprint construction of 35 medicinal Lonicera japonica germplasm resources were carried out by SSR molecular marker technology.The results showed that the average number of effective alleles and expected heterozygosity of 23 pairs of primers were 2.891 and0.618,respectively.The average Shannon information index and Nei's genetic diversity index were 1.300 and0.609,respectively.The mean value of polymorphism information content of each site was 0.597,showing a high polymorphism.At the genetic similarity coefficient of 0.250,35 tested materials were divided into four categories.In addition,the fingerprint of Lonicera japonica germplasm resources was constructed by core primer method,and the rapid identification of Lonicera japonica and the related species at the molecular level was realized.This study provides a scientific basis for distinguishing genuine and fake Lonicera japonica,enriching excellent characters and breeding high-quality varieties.

关 键 词：金银花 SSR标记 遗传多样性 指纹图谱 

分 类 号：S567.79[农业科学—中草药栽培]