巴戟天离体保存与茎尖小滴玻璃化法超低温保存体系的构建  

Construction of in Vitro Preservation and Shoot Tips Droplet-vitrification Cryopreservation System of Morinda officinalis How

作　　者：颜航 李春燕 李志英 王敏瑞 荆永琳 王小冰 李玉莲 陈浪欣 徐立[2] Yan Hang;Li Chunyan;Li Zhiying;Wang Minrui;Jing Yonglin;Wang Xiaobing;Li Yulian;Chen Langxin;Xu Li(College of Agriculture,Yangtze University,Jingzhou,434025;Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province,Key Laboratory of Chinese Herbal Medicine Biology and Cultivation(Ministry of Agriculture and Rural Affairs),Institute of Tropical Crop Genetic Resources,Chinese Academy of Tropical Agricultural Sciences,Danzhou,571737;College of Horticulture,Hainan University,Haikou,570228)

机构地区：[1]长江大学农学院,荆州434025 [2]中国热带农业科学院热带作物品种资源研究所,农业农村部中药材生物学与栽培重点实验室,海南省热带作物资源遗传改良与创新重点实验室,儋州571737 [3]海南大学园艺学院,海口570228

出　　处：《分子植物育种》2024年第19期6477-6484,共8页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2021YFD1200204);中央级公益性科研院所基本科研业务费项目(1630032022010);农业种质资源普查收集保护鉴定服务项目(19221892)共同资助。

摘　　要：本研究以巴戟天为试验材料,探究试管苗生长的最适培养容器及离体保存的最适培养基,并以此为基础构建茎尖小滴玻璃化法超低温保存体系。结果表明,组培袋中植株长势更好且延缓生长方面较玻璃瓶和塑料瓶差异不显著,可作为巴戟天离体保存培养容器。高糖低激素培养基在巴戟天离体保存方面有显著效果,试管苗的保存时间从4个月延长至6个月。以高糖低激素培养基预处理的植株茎尖经过常温玻璃法小滴玻璃化法超低温保存后再生率可达13.3%。本研究结果为巴戟天离体保存和其茎尖小玻璃化超低温保存体系的建立奠定基础。In this study,the Morinda officinalis was used as the experimental material to explore the optimal culture container for the growth of test-tube seedlings and the optimal medium for in vitro preservation.Based on this,the cryopreservation system of shoot tips droplet-vitrification was constructed.The results showed that the plant growth in the tissue culture bag was better and the growth delay was not significantly different from that in the glass bottle and the plastic bottle,which could be used as the in vitro preservation culture container of Morinda officinalis.The high sugar and low hormone medium has a significant effect on the preservation of Morinda officinalis in vitro.The preservation time of tissue culture seedlings was extended from 4 months to 6 months.The regeneration rate of shoot tips pretreated with high sugar and low hormone medium could reach 13.3%after cryopreservation by room temperature droplet-vitrification.The research results laid a foundation for the in vitro preservation of Morinda officinalis and for establishing its shoot tips droplet-vitrification cryopreservation system.

关 键 词：巴戟天 组培袋 高糖低激素培养 离体保存 小滴玻璃化法 超低温保存 

分 类 号：S567.19[农业科学—中草药栽培]