Rapid fabrication of modular 3D paper-basedmicrofluidic chips using projection-based 3D printing  

作　　者：Mingjun Xie Zexin Fu Chunfei Lu Sufan Wu Lei Pan Yong He Yi Sun Ji Wang 

机构地区：[1]Plastic and Reconstructive Surgery Center,Department of Plastic and Reconstructive Surgery,Zhejiang Provincial People’s Hospital,Affiliated People’s Hospital,Hangzhou Medical College,Hangzhou 310014,China [2]State Key Laboratory of Fluid Power and Mechatronic Systems,School of Mechanical Engineering,Zhejiang University,Hangzhou 310058,China [3]Key Laboratory of Materials Processing and Mold,Zhengzhou University,Zhengzhou 450002,China [4]Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province,College of Mechanical Engineering,Zhejiang University,Hangzhou 310058,China [5]Cancer Center,Zhejiang University,Hangzhou 310058,China

出　　处：《Bio-Design and Manufacturing》2024年第5期611-623,共13页生物设计与制造（英文）

基　　金：sponsored by the National Natural Science Foundation of China(No.52235007,YH);the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(No.T2121004,YH);the NationalNatural Science Foundation of China(No.52305300,MJX);the Fellowship of China Postdoctoral Science Foundation(No.2022M722826,MJX);the National Natural Science Foundation of China(No.82203602,JW);the Zhejiang Provincial Natural Science Foundation of China(No.LQ22H160020,JW)。

摘　　要：Paper-based microchips have different advantages,such as better biocompatibility,simple production,and easy handling,making them promising candidates for clinical diagnosis and other fields.This study describes amethod developed to fabricate modular three-dimensional(3D)paper-based microfluidic chips based on projection-based 3D printing(PBP)technology.A series of two-dimensional(2D)paper-based microfluidic modules was designed and fabricated.After evaluating the effect of exposure time on the accuracy of the flow channel,the resolution of this channel was experimentally analyzed.Furthermore,several 3D paper-based microfluidic chips were assembled based on the 2D ones using different methods,with good channel connectivity.Scaffold-based 2D and hydrogel-based 3D cell culture systems based on 3D paper-based microfluidic chips were verified to be feasible.Furthermore,by combining extrusion 3D bioprinting technology and the proposed 3D paper-based microfluidic chips,multiorgan microfluidic chips were established by directly printing 3D hydrogel structures on 3D paperbased microfluidic chips,confirming that the prepared modular 3D paper-based microfluidic chip is potentially applicable in various biomedical applications.

关 键 词：Paper-based microfluidic chip Projection-based 3D printing(PBP) Modularization Cell culture 

分 类 号：R318[医药卫生—生物医学工程]