Jetting-based bioprinting:process,dispense physics,and applications  

作　　者：Wei Long Ng Viktor Shkolnikov 

机构地区：[1]Singapore Centre for 3D Printing(SC3DP),School of Mechanical and Aerospace Engineering,Nanyang Technological University(NTU),Singapore 639798,Singapore [2]HP Inc.,Palo Alto,CA 94304,USA

出　　处：《Bio-Design and Manufacturing》2024年第5期771-799,共29页生物设计与制造（英文）

基　　金：support from NTU Presidential Postdoctoral Fellowship.

摘　　要：Jetting-based bioprinting facilitates contactless drop-on-demand deposition of subnanoliter droplets at well-defined positions to control the spatial arrangement of cells,growth factors,drugs,and biomaterials in a highly automated layer-by-layer fabrication approach.Due to its immense versatility,jetting-based bioprinting has been used for various applications,including tissue engineering and regenerative medicine,wound healing,and drug development.A lack of in-depth understanding exists in the processes that occur during jetting-based bioprinting.This review paper will comprehensively discuss the physical considerations for bioinks and printing conditions used in jetting-based bioprinting.We first present an overview of different jetting-based bioprinting techniques such as inkjet bioprinting,laser-induced forward transfer bioprinting,electrohydrodynamic jet bioprinting,acoustic bioprinting and microvalve bioprinting.Next,we provide an in-depth discussion of various considerations for bioink formulation relating to cell deposition,print chamber design,droplet formation and droplet impact.Finally,we highlight recent accomplishments in jetting-based bioprinting.We present the advantages and challenges of each method,discuss considerations relating to cell viability and protein stability,and conclude by providing insights into future directions of jetting-based bioprinting.

关 键 词：3D bioprinting BIOFABRICATION Jetting-based Dispense physics Machine learning 

分 类 号：R318[医药卫生—生物医学工程] TP391.73[医药卫生—基础医学]