不同蛋白水平饲粮中添加过瘤胃赖氨酸对牦牛生长性能、养分消化、血清生化及瘤胃发酵参数的影响  

Effects of rumen lysine supplementation in diets with different protein levels on growth performance,nutrient digestion,serum biochemistry and rumen fermentation parameters in yaks

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作  者:雷潇瑜 涂瑞 彭忠利[1] LEI Xiaoyu;TU Rui;PENG Zhongli(Laboratory of Animal Science of National Ethnic Affairs Commission,Chengdu,Sichuan Province 610041,China)

机构地区:[1]西南民族大学畜牧兽医学院,四川成都610041

出  处:《中国饲料》2024年第19期72-80,共9页China Feed

基  金:国家现代农业产业技术体系四川肉牛创新团队建设项目(sccxtd-2020-13);牦牛高效养殖关键技术与集成应用研究(20220044)。

摘  要:本试验旨在研究不同蛋白水平饲粮中添加过瘤胃赖氨酸(RPLys)对牦牛生长性能、养分表观消化率、血清生化指标及瘤胃发酵参数的影响,为将RPLys应用于牦牛养殖提供技术参考。试验采用2×3因子试验设计,分别为2种饲粮蛋白水平(低蛋白饲粮:12.98%,高蛋白饲粮:14.99%)和3种RPLys添加水平(0%、0.15%和0.30%,干物质基础)。选取36头(237.00±35.96)kg年龄为4周岁左右的健康公牦牛随机分为6组(Ⅰ组:低蛋白基础饲粮,Ⅱ组:低蛋白饲粮添加0.15%RPLys,Ⅲ组:低蛋白饲粮添加0.30%RPLys,Ⅳ组:高蛋白基础饲粮,Ⅴ组:高蛋白饲粮添加0.15%RPLys,Ⅵ组:高蛋白饲粮添加0.30%RPLys),每个处理组6个重复,每个重复1头牦牛。预饲期7 d,正饲期60 d。结果表明:(1)平均日增重在RPLys添加量主效应中以0.30%组最佳,显著高于基础饲粮组(P<0.05),在两因素交互作用中,以Ⅲ组最高,显著高于Ⅰ组(P<0.05);料重比在RPLys添加量主效应中以0.30%组最佳,显著低于基础饲粮组和0.15%组(P<0.05),在两因素交互作用中,以Ⅲ组最佳,显著高于Ⅰ组(P<0.05)。(2)在蛋白水平主效应中,高蛋白饲粮组有机物(OM)表观消化率显著低于低蛋白饲粮组,高蛋白饲粮组粗蛋白质(CP)、中性洗涤纤维(NDF)及酸性洗涤纤维(ADF)极显著低于低蛋白饲粮组(P<0.01),在两因素交互作用中,以Ⅲ组最佳,其OM、CP、NDF及ADF均显著高于Ⅰ组(P<0.05)。(3)在RPLys添加水平主效应中,添加0.15%和0.30%组丙氨酸氨基转移酶、葡萄糖、尿素、总蛋白、天门冬氨酸氨基转移酶、白蛋白、球蛋白、肌酐、胰岛素(INS)及胰岛素样生长因子1(IGF-1)相比基础饲粮组均显著升高(P<0.05),在两因素交互作用中,INS在Ⅵ组最高,显著高于Ⅰ组(P<0.05)。(4)在RPLys添加水平主效应中,添加0.15%和0.30%组游离氨基酸均显著低于基础饲粮组,在两因素交互作用中,以Ⅴ组最低,显著低于Ⅰ组(P<0.05)。(5)在蛋白水平主效应中,高蛋This experiment was conducted to investigate the effects of rumen-protected lysine(RPLys)supplementation in diets with different protein levels on growth performance,apparent nutrient digestibility,serum biochemical parameters and rumen fermentation parameters of yaks,in order to provide a technical reference for the application of RPLys in yak breeding.The experiment was conducted in a 2×3 factorial design with 2 ration protein levels(low protein ration:12.98%,high protein ration:14.99%)and 3 RPLys supplementation levels(0%,0.15%and 0.30%,dry matter basis).Thirty-six healthy male yaks with(237.00±35.96)kg,approximately 4 weeks old,were randomly divided into 6 groups(groupⅠ:low protein basal diet,groupⅡ:low protein diet with 0.15%RPLys,groupⅢ:low protein diet with 0.30%RPLys,groupⅣ:high protein basal diet,groupⅤ:high protein diet with 0.15%RPLys,groupⅥ:high protein diet with 0.30%RPLys).6 replicates per treatment group,1 yak per replicate.Pre-feeding period 7 days,regular feeding period 60 days.The results were as follows:(1)In the main effect of RPLys supplementation,the average daily gain was best in the 0.30%group,which was significantly higher than that in the basal diet group(P<0.05);in the interaction of the two factors,the highest value was found in the groupⅢ,which was significantly higher than that in the groupⅠ(P<0.05).In the main effect of RPLys supplementation,the feed to gain ratio was best in the 0.30%group,which was significantly lower than that in the basal diet group and the 0.15%group(P<0.05).In the interaction between the two factors,the ratio of feed to gain was best in the groupⅢ,which was significantly higher than that in the groupⅠ(P<0.05).(2)In the main effect of protein level,the apparent digestibility of organic matter(OM)in the high protein diet group was significantly lower than that in the low protein diet group.Crude protein(CP),neutral detergent fiber(NDF)and acid detergent fiber(ADF)in the high protein diet group were significantly lower than those in the low

关 键 词:牦牛 过瘤胃赖氨酸 蛋白水平 氨基酸 生长性能 

分 类 号:S816.7[农业科学—饲料科学]

 

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