机构地区:[1]农业微生物资源发掘与利用全国重点实验室,湖北宜昌443003 [2]湖北省酵母功能湖北省重点实验室,湖北宜昌443003
出 处:《中国饲料》2024年第19期196-202,共7页China Feed
基 金:国家重点研发计划资助(2021YFD1301002);湖北省重点研发计划项目(2023EHA061)。
摘 要:本试验旨在探究酵母培养物对湖羊体外模拟瘤胃发酵、生长性能和血常规的影响。体外模拟试验中,选择20只体况相近的湖羊作为瘤胃液的供体,对照组(CON)采用基础培养底物,试验组在基础培养底物中添加1%的酵母培养物,在体外模拟瘤胃发酵培养72 h后,测定产气量和瘤胃发酵特性参数。在动物试验中,选择品种、日龄一致,体重相近的育肥湖羊共24只,随机分为2个试验组,每组3个重复,每个重复4只羊。其中,对照组饲喂基础日粮试验组饲喂基础日粮+10 g/只·d酵母培养物,试验持续35 d。体外模拟试验结果表明:培养底物中添加酵母培养物可显著提高湖羊体外瘤胃72 h累积产气量(P<0.05),提升产气动力学指数;其次,可显著提高总挥发性脂肪酸、乙酸和丙酸浓度(P<0.05)。动物试验结果表明:饲粮中添加酵母培养物使湖羊第四周、第五周以及试验全期的干物质平均日采食量显著提高14.79%、6.03%和12.33%(P<0.05);试验组的日增重有提高的趋势(P=0.062),提高了16.75%;料肉比有降低的趋势(P=0.051),降低了11.15%;显著增加了血常规中红细胞数目和红细胞分布宽度变异系数(P<0.05)。综上所述,日粮添加1%的酵母培养物能够提高湖羊体外瘤胃发酵特性以及湖羊的生长性能和血常规指标。The aim of this experiment was to investigate the effects of yeast cultures on rumen fermentation,growth performance and blood routine of simulated rumen of lake sheep in vitro.In the in vitro simulation test,20 lake sheep with similar body condition were selected as donors of rumen fluid,the control group(CON)used basal culture substrate,and the experimental group added 1%yeast culture in basal culture substrate,and the parameters of gas production and rumen fermentation characteristics were determined after 72 h of incubation of in vitro simulated rumen fermentation.In the animal test,a total of 24 fattened lake sheep of the same age and similar weight were selected and randomly divided into 2 experimental groups,with 3 replicates in each group and 4 sheep in each replicate.The control group was fed a basal diet;the experimental group was fed a basal diet+10 g/d yeast culture,and the experiment lasted for 35 d.The results of the in vitro simulation test showed that the addition of yeast culture to the culture substrate significantly increased the cumulative gas production of the rumen of the lake sheep for 72 h in vitro(P<0.05),and elevated the kinetic index of gas production;Secondly,it significantly increased the concentrations of the total volatile fatty acids,acetic acid and propionic acid(P<0.05).The results of the animal test showed that the addition of yeast culture to the ration significantly increased the average daily intake of dry matter of lake sheep by 14.79%,6.03%and 12.33%in the fourth and fifth weeks as well as in the whole period of the test(P<0.05);there was a tendency to increase the daily weight gain of the test group(P=0.062),which was increased by 16.75%;there was a tendency to decrease the feed-to-meat ratio(P=0.051),which was decreased by 11.15%;and significantly increased the coefficient of variation of erythrocyte number and erythrocyte distribution width in blood(P<0.05).In conclusion,the addition of 1%yeast culture to the diet could improve the in vitro rumen fermentation characteris
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