基于非靶向代谢组学的云南特色丘北辣椒代谢物差异分析  

Differential Analysis of Yunnan Qiubei Chili Metabolites Based on Non⁃Targeted Metabonomics

作　　者：胡华冉 张芮豪 杜磊[1] 钟秋月[1] 刘发万[1] 李卫芬[1] 桂敏[1] HU Huaran;ZHANG Ruihao;DU Lei;ZHONG Qiuyue;LIU Fawan;LI Weifen;GUI Min(Horticultural Research Institute,Yunnan Academy of Agricultural Science,Kunming 650205,Yunnan,China)

机构地区：[1]云南省农业科学院园艺作物研究所,云南昆明650205

出　　处：《食品研究与开发》2024年第19期155-163,共9页Food Research and Development

基　　金：云南省科技厅基础研究专项(202101AU070103);国家现代农业产业技术体系项目(CARS⁃24⁃G⁃25);云南省种子种业联合实验室项目(202205AR070001);云南省发酵蔬菜重点实验室项目(202205AG070001)。

摘　　要：为探究云南特色丘北辣椒品种云干椒7号(Y7)和传统丘北辣(QB)在不同生育期的代谢产物差异,利用液相色谱⁃质谱(liquid chromatography⁃mass spectrometry,LC⁃MS)法比较分析两品种在绿熟期和红熟期的非靶向代谢物质。结果表明,代谢物的数量、组分因生育期、品种差异而表现不同。红熟期的代谢物数量明显高于绿熟期,QB中的差异代谢物显著高于Y7;两品种中特有的甘油磷脂类、黄酮类、辣椒素类以及氨基酸类等代谢物丰度较高,可以作为鉴别Y7和QB的潜在生物标志。京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析发现Y7和QB在两生育期的关键代谢通路主要富集在脂质代谢。L⁃谷氨酸、蔗糖、海藻糖等差异代谢物被注释到与果实发育、转色相关的ABC转运蛋白(ATP⁃binding cassette transporter)、次生代谢生物合成、苯丙烷代谢等通路。综上,Y7和QB的代谢成分各有优势,不仅为营养功能评价提供基础,也为干制辣椒的高品质育种与采收提供理论参考。This study explored the differences of metabolites in Yunnan Qiubei chili at different growth stages.The nontargeted metabolites from Yun dried chili No.7(Y7)and traditional Qiubei chili(QB)in green and red ripe stages were discussed respectively by liquid chromatography⁃mass spectrometry(LC⁃MS).It was found that the quantity and component varied with different growth stages and varieties.The quantity of metabo⁃lites in red ripe stage was significantly higher than that in green ripe stage,while the differential metabolites in QB exceeded those in Y7.Metabolites such as glycerophospholipids,flavonoids,capsaicin,and amino acids were abundant in the two varieties,which could be used as potential biomarkers to distinguish Y7 and QB.Ac⁃cording to the enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes(KEGG),the key meta⁃bolic pathways of Y7 and QB in the two growth stages were mainly enriched in lipid metabolism.In addition,different metabolites such as L⁃glutamate,sucrose,and trehalose were annotated into pathways related to fruit development and color transformation,including ATP⁃binding cassette transporter,secondary metabolic bio⁃synthesis,and phenylpropane metabolism.In conclusion,the metabolic components of Y7 and QB had their advantages.They provided not only the basis for nutritional function evaluation but also theoretical reference for high⁃quality breeding and harvesting of dried chili.

关 键 词：云南丘北辣椒 液相色谱⁃质谱(LC⁃MS) 非靶向代谢组 差异代谢物 生育期 

分 类 号：S641.3[农业科学—蔬菜学]