Anti-infective immune functions of type Ⅳ interferon in grass carp(Ctenopharyngodon idella):A novel antibacterial and antiviral interferon in lower vertebrates  

作　　者：Yuchen Liu Wentao Zhu Yanqi Zhang Jingjing Zhang Maolin Lv Jianguo Su 

机构地区：[1]Hubei Hongshan Laboratory,College of Fisheries,Huazhong Agricultural University,Wuhan,Hubei 430070,China [2]Laboratory for Marine Biology and Biotechnology,Qingdao Marine Science and Technology Center,Qingdao,Shandong 266237,China

出　　处：《Zoological Research》2024年第5期972-982,共11页动物学研究（英文）

基　　金：supported by the Biological Breeding-Major Projects(2023ZD04065)。

摘　　要：Type Ⅳ interferon(IFN-υ)is a recently discovered cytokine crucial for host defense against viral infections.However,the role and mechanisms of IFN-υin bacterial infections remain unexplored.This study investigated the antibacterial and antiviral functions and mechanisms of grass carp(Ctenopharyngodon idella)IFN-υ(CiIFN-υ)both in vivo and in vitro.The CiIFN-υgene was first identified and characterized in grass carp.Subsequently,the immune expression of CiIFN-υsignificantly increased following bacterial challenge,indicating its response to bacterial infections.The eukaryotic recombinant expression plasmid of CiIFN-υwas then constructed and transfected into fathead minnow(FHM)cells.Supernatants were collected and incubated with four bacterial strains,followed by plate spreading and colony counting.Results indicated that CiIFN-υexhibited more potent antibacterial activity against gram-negative bacteria compared to gram-positive bacteria and aggregated gram-negative bacteria but not gram-positive bacteria.In vivo experiments further confirmed the antibacterial function,showing high survival rates,low tissue edema and damage,reduced tissue bacterial load,and elevated proinflammatory response at the early stages of bacterial infection.In addition,the antiviral function of CiIFN-υwas confirmed through in vitro and in vivo experiments,including crystal violet staining,survival rates,tissue viral burden,and reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR).This study highlights the antibacterial function and preliminary mechanism of IFN-υ,demonstrating that IFN-υpossesses dual functions against bacterial and viral infections.

关 键 词：Grass carp(Ctenopharyngodon idella) IFN-υ Bactericidal activity Antiviral activity Antimicrobial immunity 

分 类 号：S943[农业科学—水产养殖]