基于网络药理学探讨《种福堂公选良方》辟瘟丹预防新型冠状病毒感染作用机制  

Discussion on the Mechanism of Biwen Pills from Zhong Fu Tang Gong Xuan Liang Fang in Preventing COVID-19 Based on Network Pharmacology

作　　者：吴俊仪 肖静 方艳 张伟娜[2] 赵静[3] 吴夏秋[1] WU Junyi;XIAO Jing;FANG Yan;ZHANG Weina;ZHAO Jing;WU Xiaqiu(Public Health,Zhejiang Chinese Medical University,Hangzhou 310053,China;Institute of Information on Traditional Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Basic Research in Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]浙江中医药大学公共卫生学院,浙江杭州310053 [2]中国中医科学院中医药信息研究所,北京100700 [3]中国中医科学院中医临床基础医学研究所,北京100700

出　　处：《中国中医药图书情报杂志》2024年第5期53-57,共5页Chinese Journal of Library and Information Science for Traditional Chinese Medicine

基　　金：中国中医科学院科技创新工程(CI2021A00704-4);中国中医科学院基本科研业务自主选题项目(ZZ11-057);2022年国家级大学生创新创业训练计划(202210344038)。

摘　　要：目的运用网络药理学方法探讨辟瘟丹预防新型冠状病毒感染(COVID-19)的作用机制。方法通过TCMSP数据库检索辟瘟丹的化学成分及作用靶点;通过GeneCards、OMIM、PharmGKB、TTD和DrugBank数据库查询COVID-19的相关靶点;借助STRING网站进行蛋白质互相作用分析;利用Cytoscape3.9.0软件构建药物成分-靶点网络,分析获得辟瘟丹预防COVID-19的核心靶点;利用R语言和KOBAS数据库分别进行GO和KEGG富集分析。结果辟瘟丹化学成分共115个,靶点共2378个,辟瘟丹和COVID-19有重叠靶点101个,核心靶点为TP53、HSP90AA1、AKT1、MAPK3;KEGG富集分析得到信号通路102条,主要涉及IL-17信号通路、TNF信号通路、P53信号通路、AGE-RAGE信号通路等;GO分析后共得到生物学过程2359个、细胞组成423个、分子功能161个。结论辟瘟丹能够通过TP53、HSP90AA1、AKT1、MAPK3靶点,IL-17、TNF、P53、AGE-RAGE信号通路途径达到调节细胞凋亡、促进机体免疫、抗病毒等作用,发挥预防COVID-19的药理作用。Objective To explore the mechanism of Biwen Pills in preventing COVID-19 based on network pharmacology.Methods The chemical components and targets of action of Biwen Pills were retrieved through the TCMSP database;the disease targets were screened by searching the GeneCards,OMIM,PharmGKB,TTD and DrugBank databases.Then STRING was applied to build the network of protein-protein interaction network(PPI).Cytoscape 3.9.0 software was used to build the“drug-target”network diagram and core targets of Biwen Pills in preventing COVID-19 were obtained.The R language and KOBAS database was used to perform GO and KEGG enrichment analysis.Results There were 115 chemical components in Biwen Pills,with a total of 2378 target points.There were 101 overlapping targets between Biwen Pills and COVID-19,with the main core targets being TP53,HSP90AA1,AKT1,and MAPK3;102 signaling pathways were obtained from KEGG analysis,mainly involving IL-17 signaling pathway,TNF signaling pathway,p53 signaling pathway,AGE-RAGE signaling pathway,etc;after GO analysis,a total of 2359 biological processes,423 cell compositions,and 161 molecular functions were obtained.Conclusion Biwen Pills can regulate apoptosis,promote immunity and anti-virus through TP53,HSP90AA1,AKT1,MAPK3 targets,IL-17,TNF,P53,AGE-RAGE signaling pathways,and exert pharmacological therapeutic effects in preventing COVID-19.

关 键 词：辟瘟丹 新冠病毒 网络药理学 

分 类 号：R285[医药卫生—中药学]