88份四川省小麦后备品系抗条锈性评价及抗性基因的分子检测  

Evaluation of resistance to stripe rust and analysis of Yr resistance genes of 88 wheat backup lines in Sichuan Province

作　　者：张兴宗 黄亮 夏先全[4] 张华 陈万权[2,3] 夏崇靖 刘太国 Zhang Xingzong;Huang Liang;Xia Xianquan;Zhang Hua;Chen Wanquan;Xia Chongjing;Liu Taiguo(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,Sichuan Province,China;State Key Laboratory for Biology of Plant Disease and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Science,Beijing 100193,China;National Agricultural Experimental Station for Plant Protection at Gangu,Ministry of Agriculture and Rural Affairs,Tianshui 741000,Gansu Province,China;Institute of Plant Protection,Sichuan Academy of Agricultural Sciences,Chengdu 610023,Sichuan Province,China;Crop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province,Mianyang Academy of Agricultural Sciences,Mianyang 621023,Sichuan Province,China)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]中国农业科学院植物保护研究所,植物病虫害综合治理全国重点实验室,北京100193 [3]农业农村部国家植物保护甘谷观测实验站,甘肃天水741000 [4]四川省农业科学院植物保护研究所,成都610023 [5]绵阳市农业科学研究院,厅市共建作物特色资源创制及应用四川省重点实验室,四川绵阳621023

出　　处：《植物保护学报》2024年第4期764-773,共10页Journal of Plant Protection

基　　金：国家重点研发计划(2021YFD1401000);国家小麦产业技术体系(CARS-3);中国农业科技创新工程(CAAS-ZDRW202405)。

摘　　要：为了解四川省小麦Triticum aestivum育成品系对当前主流小麦条锈菌Puccinia striiformis f.sp.tritici生理小种的抗性水平和所含抗性基因情况,利用当前主流生理小种CYR32和CYR34对88份四川省后备小麦品系进行苗期抗性鉴定,并通过7个已知条锈病抗性基因Yr5、Yr9、Yr10、Yr15、Yr17、Yr18和Yr26对其所含重要抗性基因进行分子标记检测。结果表明:在苗期有85份小麦品系对CYR32表现为抗病,有86份对CYR34表现为抗病,分别占供试品系总数的96.59%和97.73%,其中川麦815和川麦809均对CYR32和CYR34表现为感病。在85份后备小麦品系中检测到抗性基因,其中有35份携带Yr5,有4份携带Yr9,有1份携带Yr10,有50份携带Yr15,有50份携带Yr17,有1份携带Yr18,有36份携带Yr26;仅川麦815、蜀糯麦2284和蜀麦2255未检测到上述7个抗性基因;抗性基因聚合分析结果显示,共有66份小麦品系携带2个及以上抗性基因,表明四川省后备小麦品系利用抗条锈病基因聚合育种的比例较高。In order to identify the resistance levels of wheat lines in Sichuan Province to stripe rust pathogen Puccinia striiformis f.sp.tritici,88 newly developed wheat lines were evaluated at seeding stage by using two prevalent races CYR32 and CYR34.Meanwhile,the panel of wheat lines were detected with functional markers or closely linked molecular markers of the resistance genes Yr5,Yr9,Yr10,Yr15,Yr17,Yr18 and Yr26.The results showed that 85 wheat lines(96.59%)were resistant to the race CYR32 and 86 lines(97.73%)were resistant to CYR34 at seedling stage.Chuanmai 815 and Chuanmai 809 were susceptible to both CYR32 and CYR34.Among the 85 Sichuan backup wheat lines carrying resistance genes,35 lines carried Yr5,four lines with Yr9,one line with Yr10,50 lines with Yr15,50 lines with Yr17,one line with Yr18,and 36 lines with Yr26.The seven stripe rust resistance genes was not detected in wheat lines Chuanmai 815,Shunnuomai 2284 and Shumai 2255.The analysis of resistance gene pyramiding showed that 66 wheat lines carried two or more resistance genes,indicating that the proportion of backup wheat lines was higher in Sichuan Province using resistance gene pyramiding breeding.

关 键 词：小麦品系 条锈病 抗性评价 Yr基因 分子检测 

分 类 号：S512.1[农业科学—作物学]