取食抗感品种紫花苜蓿的苜蓿斑蚜唾液腺转录组差异分析  

Analysis of spotted alfalfa aphid Therioaphis trifolii salivary gland transcriptomedifferences based on feeding on susceptible and resistant varieties of Medicago sativa

作　　者：古文鑫 朱凯辉 刘长仲 涂雄兵[2] Gu Wenxin;Zhu Kaihui;Liu Changzhong;Tu Xiongbing(College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,Gansu Province,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]甘肃农业大学植物保护学院,兰州730070 [2]中国农业科学院植物保护研究所,植物病虫害综合治理全国重点实验室,北京100193

出　　处：《植物保护学报》2024年第4期899-909,共11页Journal of Plant Protection

基　　金：财政部和农业农村部国家现代农业产业技术体系(CARS-34-07)。

摘　　要：为明确苜蓿斑蚜Therioaphis trifolii在不同品种紫花苜蓿Medicago sativa上适合度存在差异的原因,收集经抗虫品种与感虫品种紫花苜蓿驯化得到的2个苜蓿斑蚜抗性品系与感性品系的唾液腺,采用Illumina二代测序平台进行转录组测序,对测序所得序列进行注释分析并筛选差异表达基因,从中随机挑选8个下调差异表达基因进行实时荧光定量PCR(real-time quantitative PCR,RTqPCR)验证。结果显示:在苜蓿斑蚜唾液腺样本中共鉴定到2 292个差异表达基因,以感性品系唾液腺基因为对照,其中上调表达基因有1 191个,下调表达基因有1 101个,主要包括营养代谢、信号通路转导和糖代谢相关酶合成相关基因,其中负责糖蛋白、糖脂和寡糖降解的岩藻糖苷酶合成基因和气味识别基因下调表达,表明此类基因可能参与苜蓿斑蚜应对寄主植物抗性的反防御过程。8个下调差异表达基因的RT-qPCR测定结果与转录组数据分析结果趋势一致,说明转录组数据可靠。推测对抗虫品种紫花苜蓿有更强适应性的苜蓿斑蚜抗性品系的唾液腺中的活跃基因可能是参与二者互作的关键因子。To clarify the reasons for the differences in fitness of the spotted alfalfa aphid,Therioaphis trifolii,on different varieties of alfalfa,Medicago sativa,salivary glands were collected from two strains of alfalfa spotted aphids:one resistant and one susceptible.These strains had been domesticated from aphid-resistant and susceptible varieties of alfalfa.Transcriptome sequencing was performed using the Illumina second-generation sequencing platform.The sequences were annotated and analyzed to screen differentially expressed genes.Subsequently,eight randomly selected down-regulated differential genes were validated using real-time quantitative PCR(RT-qPCR).The results identified a total of 2292 differentially expressed genes in the salivary gland samples of the alfalfa spotted aphid,with 1191 genes up-regulated and 1101 genes down-regulated in the salivary glands of the susceptible strain.These genes mainly included those related to nutrient metabolism,signaling pathway transduction,and the synthesis of enzymes related to glucose metabolism.Notably,genes for the degradation of glycoproteins,glycolipids,oligosaccharides,fucosidase synthesis,and odor recognition were down-regulated.This suggests that these genes might be involved in the counter-defense processes of the alfalfa aphid in response to host plant resistance.The RT-qPCR assay results for the eight differential genes demonstrated a consistent pattern with the transcriptome analysis results,indicating the reliability of the data.It can be hypothesized that the active genes in the salivary glands of the alfalfa spotted aphid,which show greater adaptability to insect-resistant alfalfa,may be key factors involved in the interaction between plants and insects.

关 键 词：紫花苜蓿 苜蓿斑蚜 唾液腺 转录组 差异表达基因 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]