Exendin-4对小鼠黑质网状部神经元自发放电影响  

Effect of Exendin-4 on the spontaneous discharge of neurons in the substantia nigra pars reticulata of mice

作　　者：沈方帅 李筱雪 刘翠 陈心怡 薛雁 陈蕾 SHEN Fangshuai;LI Xiaoxue;LIU Cui;CHEN Xinyi;XUE Yan;CHEN Lei(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)

机构地区：[1]青岛大学医学部基础医学院生理学与病理生理学系,山东青岛266071 [2]青岛大学附属医院国际医疗中心,山东青岛266071

出　　处：《青岛大学学报（医学版）》2024年第4期491-495,共5页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金青年科学基金项目(32200939);山东省自然科学基金资助项目(ZR2022QC225,ZR2023MC072);泰山学者青年专家计划资助项目(tsqnz20230634)。

摘　　要：目的探究胰高血糖素样肽-1(GLP-1)受体激动剂Exendin-4对小鼠黑质网状部神经元自发放电的影响。方法采用免疫荧光染色观察GLP-1受体(GLP-1R)在小鼠黑质网状部的表达。使用在体电生理微电极注射10μmol/L的Exendin-4到小鼠黑质网状部神经元表面,观察黑质网状部非多巴胺能(假定为γ-氨基丁酸能)神经元的自发放电变化。结果免疫荧光染色结果显示,小鼠黑质网状部有GLP-1R的高表达。在所记录到的13个黑质网状部神经元中,Exendin-4可显著降低其中9个神经元的自发放电,其放电频率由加药前(18.62±3.19)Hz降低到(14.615±3.06)Hz,差异有显著性(t=7.257,P<0.05);其放电频率平均降低(21.85±7.72)%,较生理盐水组的(1.15±9.67)%显著升高(Z=2.282,P<0.05)。在另外的4个黑质网状部神经元中,Exendin-4对放电频率无明显影响,其放电频率由(13.49±7.16)Hz变为(13.54±7.45)Hz,差异无显著性。结论Exendin-4能降低部分黑质网状部神经元的自发放电频率。Objective To investigate the effectof Exendin-4,a glucagon-like peptide-1(GLP-1)receptor agonist,on the spontaneous discharge of neurons in the substantia nigra pars reticulate of mice.Methods Immunofluorescent staining was used to observe the expression of GLP-1receptor(GLP-1R)in the substantia nigra pars reticulata of mice.Extendin-410μmol/L were injected onto the surface of neurons in the substantia nigra pars reticulate using in vivo electrophysiological microelectrodes to observe the spontaneous discharge of non-dopaminergic neurons(assumed to be GABAergic neurons)in the substantia nigra pars reticulate.Results Immunofluorescent staining showed a high expression level of GLP-1R in the substantia nigra par reticulate of mice.As for the 13 neurons recorded in the substantia nigra par reticulata,Exendin-4 significantly reduced the spontaneous discharge rate of 9 neurons,with a significant reduction in discharge frequency from(18.62±3.19)Hz to(14.615±3.06)Hz(t=7.257,P<0.05),and the discharge frequency was reduced by(21.85±7.72)%on average,which was significantly higher than the reduction rate of(1.15±9.67)%in the normal saline group(Z=2.282,P<0.05).In the other 4 neurons,Exendin-4 had no signi-ficant effect on discharge frequency,which changed from(13.49±7.16)Hz to(13.54±7.45)Hz.Conclusion Exendin-4 can reduce the spontaneous discharge frequency of some neurons in the substantia nigra pars reticulata.

关 键 词：艾塞那肽 黑质 网状部 胰高血糖素样肽-1受体 电生理学 小鼠 近交C57BL 

分 类 号：R977.15[医药卫生—药品] R322.81[医药卫生—药学]