福建霞浦茶树炭疽菌的分离鉴定与防治药物筛选  

Isolation,identification and fungicides screening of the pathogen of tea plant anthracnose in Xiapu County of Fujian Province

作　　者：张承康 郭田龙 王学彧 卢赐鼎 李贺 ZHANG Cheng-kang;GUO Tian-long;WANG Xue-yu;LU Ci-ding;LI He(College of Biological Science and Engineering,Ningde Normal University,Ningde,Fujian 352100,China;Agriculture and Rural Bureau of Xiapu County,Ningde,Fujian 355100,China;Forestry College,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Technology Center of Fuzhou Customs District,Fuzhou,Fujian 350000,China)

机构地区：[1]宁德师范学院生物科学与工程学院,福建宁德352100 [2]霞浦县农业农村局,福建宁德355100 [3]福建农林大学林学院,福建福州350002 [4]福州海关技术中心,福建福州350000

出　　处：《宁德师范学院学报（自然科学版）》2024年第3期283-291,共9页Journal of Ningde Normal University(Natural Science)

基　　金：国家自然科学基金项目(32001853);宁德师范学院科研项目(2022ZX405)。

摘　　要：通过组织分离法对福建省霞浦县‘福云6号’茶树病叶进行病原菌分离、纯化、回接和多基因分子鉴定,并通过农药胁迫鉴定抑菌效果.结果表明:病叶表面具灰白色病斑,病健分界线清晰,呈棕褐色,病斑上有许多分生孢子盘.病原菌在PDAY培养基培养4 d后,产生直径4.0 cm的菌落,菌落表面是密集绒毛状、灰白色菌丝,背面呈橘黄色色素,并带有点状黑色素沉积.经刮除气生菌丝并光照24 h后,菌落可产生橙色油润的分生孢子群,分生孢子呈近似圆柱状,中间直两端半圆,无隔膜,并可在疏水表面形成黑色附着胞.进一步基于ITS、ACT、CAL和GAPDH基因序列构建多基因系统发育树,明确该病原菌为果生炭疽菌(Colletotrichum fructicola).4种农药的胁迫实验表明,百菌清和吡唑醚菌酯的抑菌效果好,多菌灵则无抑菌效果.Tea plant anthracnose is an important factor affecting the yield and quality of tea in Xiapu County,Fujian Province.Identifying the pathogen responsible for the disease is conducive to the targeted control.The pathogens were isolated and purified from Fuyun 6 tea tree disease leaves in Xiapu County,Fujian Province by tissue separation and polygenic molecular identification,and the fungicide inhibition rate was determined.The results showed that:there were grayish white spots on the surface of the diseased leaves,and the brown boundary line between sickness and health parts was clear.There were many acervulus on the spots.After cultured on PDAY medium for 4 d,the pathogen produced a colony with a diameter of 4.0 cm,with dense,fluffy and grayish-white mycelia on the surface of the colony,orange pigment at the back with punctured melanin deposition.After the removal of aerial hyphae and illumination,the colonies can produce orange,oily conidial clusters.The conidia are close to cylindrical in shape,straight in the middle and semicircular at both ends.They lack septum,and may form black appressoria on the hydrophobic surface.The pathogen was identified as Colletotrichum fructicola by further multigene phylogenetic tree constructed based on ITS,ACT,CAL and GAPDH gene sequences.The stress tests of different fungicides showed that the inhibition effect of chlorothalonil and pyrazolium were good,whereas carbendazim had no inhibitory effect.

关 键 词：茶树炭疽病 分离鉴定 农药防治 ‘福云6号’茶树 霞浦县 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]