菊花茎叶清除自由基动力学特性及抗氧化活性成分的在线筛选  

Antioxidant Kinetic Characteristics and Online Identification of Active Compounds in Chrysanthemi Flos Stems and Leaves

作　　者：屠万倩 卢新 张留记 刘一菲[1] 王建霞 李向阳 陈晶晶 TU Wanqian;LU Xin;ZHANG Liuji;LIU Yifei;WANG Jianxia;LI Xiangyang;CHEN Jingjing(Henan Integrated Hospital of Traditional Chinese Medicine and Western Medicine,Henan Engineering Center of Genuine Medicinal Materials Comprehensive Development,Zhengzhou 450004,China;Henan University of Chinese medicine,Zhengzhou 450008,China;Henan Health Care College,Zhengzhou 450008,China;Henan Institute for Drug and Medical Device Inspection,Henan Vaccine Issuance Center,NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine,Chinese Materia Medica and Prepared Slices,Zhengzhou 450018,China)

机构地区：[1]河南省中西医结合医院河南省道地药材综合开发工程研究中心,郑州450004 [2]河南中医药大学,郑州450008 [3]河南卫生健康干部学院,郑州450008 [4]河南省药品医疗器械检验院河南省疫苗批签中心、国家药品监督管理局中药材及饮片质量控制重点实验室,郑州450018

出　　处：《世界科学技术-中医药现代化》2024年第7期1862-1869,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：河南省科学技术厅工程技术研究中心建设项目(2020338):河南省道地药材综合开发工程研究中心建设项目,负责人:张留记。

摘　　要：目的本研究对菊花茎叶抗氧化活性和清除自由基动力学特性进行考察,在线筛选与清除自由基相关的活性成分。方法采用DPPH法和ABTS法观察菊花茎叶提取物的抗氧化活性,比较了样品浓度、反应温度和反应时间等影响因素对自由基清除率的影响,测定了菊花茎叶清除自由基活性的半数清除率(IC50)。以木犀草素葡萄糖醛酸苷、木犀草苷和咖啡酸等黄酮成分、绿原酸、新绿原酸、隐绿原酸、异绿原酸A、B、C等酚酸成分为指标,采用HPLC法对反应前后菊花茎叶进行了含量测定,计算上述成分的消耗率。结果菊花茎叶的样品浓度、反应温度和反应时间均对其对清除DPPH自由基和ABTS自由基清除率有影响;菊花茎叶清除DPPH和ABTS自由基的IC50分别为0.395 mg·mL^(-1)、2.039 mg·mL^(-1)。与DPPH和ABTS自由基反应后,菊花茎叶样品中上述测定成分的含量均明显下降,推测上述成分可能是菊花茎叶抗氧化的活性成分。结论菊花茎叶具有较好的清除自由基活性,本研究初步明确其动力学特性,在线筛选出了抗氧化活性成分,为今后菊花茎叶的综合开发提供实验依据。Objective To study the antioxidant activity and free radical scavenging kinetics of Chrysanthemi flos stems and leaves,and online screen the active components related to free radical scavenging.Methods Using DPPH method and ABTS method the antioxidant activity of extracts from Chrysanthemi flos stems and leaves was observed,and the effects of different influencing factors,such as drug concentration,reaction temperature and reaction time on free radical scavenging rate were compared,the half scavenging rate(IC50)was determined.The flavonoids,such as luteolin glucuronic acid,luteolin,and caffeic acid,phenolic acids such as chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,B,and C in the samples before and after the reaction were chosen as indexes,and their contents were determined by HPLC.Results The drug concentration,reaction temperature and reaction time could affect the scavenging rate of DPPH free radical and ABTS.The IC50 of DPPH and ABTS were 0.395 mg·mL^(-1) and 2.039 mg·mL^(-1) respectively.After reaction with DPPH and ABTS,the contents of the analytes above all decreased significantly,which suggested that,the components above might be the antioxidant components in Chrysanthemi flos stems and leaves.Conclusions Chrysanthemi flos Stems and leaves have good free radical scavenging activity.In this study,the kinetic characteristics of scavenging DPPH and ABTS free radicals in Chrysanthemi flos stems and leaves were preliminarily clarified,and the active components were found out.It provides an experimental basis for the future comprehensive development of Chrysanthemi flos stems and leaves.

关 键 词：菊花茎叶 自由基 动力学特征 抗氧化 活性成分 HPLC 

分 类 号：R284.1[医药卫生—中药学]