水稻褐变穗病病原鉴定及其生物学特性分析  

Identification and biological characteristics of rice brown panicle disease

作　　者：杜娟[1] 曹季禺 杨瑞 哈孜拉·赛依里汗 姚兆群[1] 张学坤[1] 张莉[1] DU Juan;CAO Jiyu;YANG Rui;HAIZILA·Saiyilihan;YAO Zhaoqun;ZHANG Xuekun;ZHANG Li(College of Agriculture,Shihezi University/Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization of Xinjiang Uygur Autonomous Region,Shihezi 832003,China;Agricultural Technology Extension Station of the First Division of Xinjiang Production and Construction Corps,Alaer 843300,China)

机构地区：[1]石河子大学农学院/新疆绿洲农业病虫害治理与植保资源利用重点实验室,新疆石河子832003 [2]新疆生产兵团第一师农业技术推广站,新疆阿拉尔843300

出　　处：《安徽农学通报》2024年第19期80-85,共6页Anhui Agricultural Science Bulletin

摘　　要：对石河子大学农学院试验基地水稻病穗进行分离和纯化,通过形态学、核糖体DNA内转录间隔区(rDNA-ITS)和组蛋白3基因序列对分离病原物进行分析及致病性测定,并研究其生物学特性。结果表明,引起水稻穗部褐色病斑的病原为链格孢菌(Alternaria alternata),通过致病性测定,分离菌株均可导致水稻穗部出现与采样稻穗类似的症状,且从发病的水稻穗部分离出同样的链格孢菌。该病原菌最适生长培养基是以蔗糖为碳源的培养基,最适pH 5~7,最适生长温度29~32℃,最适生长光照24 h全光。综上,本试验中水稻褐变穗病是由链格孢菌(A.alternata)引起。The diseased panicles of rice was isolated and purified in the experimental base of College of Agricultural,Shihezi University.The isolated pathogens was analyzed by morphology,ribosomal DNA internal transcribed spacers(rDNA-its)and histone 3 gene sequences,and the pathogenicity was determined,and its biological characteristics was studied.The results showed that Alternaria alternata was the pathogen causing brown lesions on rice panicles.The pathogenicity test showed that the rice panicles had the same symptoms as the sampled rice panicles,and the same Alternaria was isolated from the diseased panicle of rice.The optimum medium for the growth of the pathogen was sucrose as carbon source.The optimum pH 5-7.The optimum growth temperature 29-32℃.The optimum growth light 24 h full light.In conclusion,the rice brown panicle disease was caused by Alternaria alternata.

关 键 词：水稻褐变穗 链格孢菌 形态学鉴定 分子生物学鉴定 生物学特性 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]