浅表血栓性静脉炎管壁基质金属蛋白酶及其抑制剂表达研究  

Expression of matrix metalloproteinases and their tissue inhibitors in the vein wall following superficial venous thrombosis

作　　者：李星月[1] 董伟 徐永波 张文法 胡晓璇 钟玉绪 褚海波 Li Xingyue;Dong Wei;Xu Yongbo;Zhang Wenfa;Hu Xiaoxuan;Zhong Yuxu;Chu Haibo(Department of General Surgery,Affiliated Hospital of Weifang Medical College,Weifang 261035,China;Department of General Surgery,Jiaozhou Branch of Shanghai East Hospital,Tongji University,Qingdao 266300,China;Department of General Surgery,the 80th Group Army Hospital of People’s Liberation Army,Weifang 261200,China;State Key Laboratory of Toxicology and Medical Countermeasures,Institute of Pharmacology and Toxicology,Beijing 100850,China)

机构地区：[1]潍坊医学院附属医院普通外科,潍坊261035 [2]同济大学附属东方医院胶州医院普通外科,青岛266300 [3]中国人民解放军陆军第80集团军医院普通外科,潍坊261200 [4]中国人民解放军军事科学院军事医学研究院毒物药物研究所,北京100850

出　　处：《中华普通外科学文献（电子版）》2024年第5期338-343,共6页Chinese Archives of General Surgery(Electronic Edition)

基　　金：中国人民解放军后勤重大项目(AWS17J008);潍坊市青年医学人才托举工程基金。

摘　　要：目的检测浅表血栓性静脉炎(SVT)、大隐静脉曲张(VGSVs)和正常大隐静脉(GSVs)管壁基质金属蛋白酶(MMPs)和其组织抑制剂(TIMPs)表达变化。方法应用免疫组织化学(IHC)、聚合酶链反应(PCR)、Western blotting方法,检测三组静脉管壁中MMP-2、MMP-9、TIMP-1、TIMP-2蛋白阳性表达率、mRNA和蛋白表达。结果SVT组MMP-2、MMP-9、TIMP-1、TIMP-2蛋白阳性表达率、mRNA和蛋白表达均显著高于VGSVs组和GSVs组,差异有统计学意义(P<0.05)。MMP-2/TIMP-2比值SVT组和VGSVs组均低于GSVs组,差异有统计学意义(P<0.05);MMP-9/TIMP-1比值SVT组均低于VGSVs组和GSVs组,差异有统计学意义(P<0.05)。结论在高流体静力压和炎症条件下,SVT管壁MMPs和TIMPs蛋白表达明显失调,提示MMPs和TIMPs可能参与静脉管壁重塑过程。Objective To investigate the changes in expression of matrix metalloproteinases(MMPs)and the tissue inhibitor of metalloproteinases(TIMPs)from superficial venous thrombosis(SVT),varicose great saphenous veins(VGSVs),and great saphenous veins(GSVs).Methods In the venous walls of the three groups,the expression of MMP-2,MMP-9,TIMP-1,and TIMP-2 proteins,protein-positive expression ratios,mRNA expression,and protein expression were determined by immunohistochemistry,polymerase chain reaction,and Western blotting.Results The MMP-2,MMP-9,TIMP-1,and TIMP-2 protein-positive expression ratios,mRNA and protein expression in the SVT group were significantly higher than those in the VGSVs and the GSVs groups(P<0.05).The MMP-2/TIMP-2 ratios in the SVT and the VGSVs groups were significantly lower than those in the GSVs group(P<0.05),respectively.The MMP-2/TIMP-2 and MMP-9/TIMP-1 ratios in the SVT group were significantly lower than those in the VGSVs and the GSVs groups(P<0.05),respectively.Conclusions Disequilibrium of MMPs and TIMPs in SVT wall occurs due to underlying high hydrostatic pressure and inflammation.These results suggested that MMPs and TIMPs participate in the process of venous wall remodeling.

关 键 词：浅表血栓性静脉炎 隐静脉 基质金属蛋白酶 金属蛋白酶组织抑制剂 

分 类 号：R654[医药卫生—外科学]