中度与重度寻常痤疮患者非皮损区毛囊菌群分析:一项单中心横断面研究  

Analysis of hair follicle microbiota in non-lesional areas of patients with moderate-tosevere acne vulgaris:a single-center cross-sectional study

作　　者：梁梦晨 李嘉祺 吴心怡 莫小辉 鞠强[1] LIANG Mengchen;LI Jiaqi;WU Xinyi;MO Xiaohui;JU Qiang(Department of Dermatology,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China)

机构地区：[1]上海交通大学医学院附属仁济医院皮肤科,上海200127

出　　处：《上海交通大学学报（医学版）》2024年第9期1094-1103,共10页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(82373506);上海交通大学医学院附属仁济医院课题(IIT-2022-0011)。

摘　　要：目的·研究中度、重度寻常痤疮患者与健康者非皮损区毛囊菌群群落结构和载量的差异,探讨微生物与寻常痤疮及其严重程度之间的关系。方法·采用横断面研究,选取2022年8月—2023年8月在上海交通大学医学院附属仁济医院皮肤科就诊的中度、重度寻常痤疮(简称痤疮)患者和健康志愿者。取中度、重度痤疮患者及健康志愿者面部非皮损区毛囊内容物进行16S rRNA高通量测序以及实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qPCR)检测,分析不同严重程度痤疮患者毛囊细菌菌群多样性、物种组成差异和菌群载量差异。结果·纳入10例中度痤疮患者、11例重度痤疮患者及11例健康志愿者,3组间年龄、性别等一般资料差异均无统计学意义。中度痤疮组和重度痤疮组相对于健康组细菌α多样性均显著下降(P=0.020,P=0.013)。主坐标分析(principal coordinates analysis,PCoA)图表明健康组人群样本分布较为集中,组内差异较小,而中度痤疮组及重度痤疮组人群样本分布有一定的趋势但较为离散,组内之间存在较大差异。3组样本趋势分布差异明显,组间微生物群落结构差异较大。相似性分析结果显示健康组与中度痤疮组(P=0.027)、健康组与重度痤疮组(P=0.017)组间β多样性有显著差异,物种组成相似度低。中度痤疮组与重度痤疮组(P=0.160)组间物种相似度较高。门水平上3组的优势菌群均为放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)以及拟杆菌门(Bacteroidetes)。属水平上健康组优势菌为丙酸杆菌属(Propionibacterium)、未分类的放线菌属(unclassified Actinomycetales),2组痤疮组优势菌均为葡萄球菌属(Staphylococcus)、丙酸杆菌属。相对于健康组,中度痤疮组和重度痤疮组非皮损区毛囊中的葡萄球菌属相对丰度均显著增加(P=0.010,P=0.019),毛囊菌群载量均显著增加(均P=0.0Objective·To study the differences in the structure and load of hair follicle microbiota in non-lesional areas among patients with moderate-to-severe acne vulgaris and healthy individuals,and to explore the relationship between microorganisms and the severity of acne vulgaris.Method·A cross-sectional study was used.Patients with moderate or severe acne vulgaris(referred to as acne)and healthy volunteers who visited the Department of Dermatology,Renji Hospital,Shanghai Jiao Tong University School of Medicine,from August 2022 to August 2023.16S rRNA high-throughput sequencing and quantitative real-time polymerase chain reaction(qPCR)were performed on the follicular contents from the non-lesional areas of the faces of patients with moderate or severe acne and healthy volunteers to analyze the diversity,species composition,and microbial load differences in hair follicle bacteria in patients with different severity of acne.Results·Ten patients with moderate acne,eleven patients with severe acne,and eleven healthy volunteers were included.There were no statistically differences in general data such as age and gender ratio among the three groups.Bacterialα-diversity was significantly lower in both the moderate and severe acne groups compared to the healthy group(P=0.020,P=0.013).The principal coordinates analysis(PCoA)plot showed that the sample distribution of the healthy group was relatively concentrated,with small differences within the group,and the distribution of samples in the moderate and severe acne groups exhibited a certain trend but was relatively scattered,with differences between the groups.There were differences in the trend distribution of the three sample groups,and there were differences in the microbial community structure between the groups.The results of similarity analysis showed significant differences inβ-diversity and low similarity in species composition between the healthy and moderate acne groups(P=0.027)and between the healthy and severe acne groups(P=0.017),and high species similarity

关 键 词：寻常痤疮 毛囊菌群 葡萄球菌属 细菌载量 

分 类 号：R751[医药卫生—皮肤病学与性病学]