通关藤对胃癌小鼠Src/FAK信号通路及Th1/Th2相关因子水平的影响  

Effects of Marsdeniae Tenacissimae Caulis on Src/FAK Signaling Pathway and Th1/Th2-Related Cytokine Levels in Mice with Gastric Cancer

作　　者：姬安龙 曾祥勇 刘彦合 傅祥炜 JI An-long;ZENG Xiang-yong;LIU Yan-he;FU Xiang-wei(Department of General Surgery,The Second Afiliated Hospital of Hainan Medical University,Haikou Hainan 570311)

机构地区：[1]海南医学院第二附属医院普通外科,海南海口570311

出　　处：《世界中西医结合杂志》2024年第9期1760-1765,共6页World Journal of Integrated Traditional and Western Medicine

基　　金：海南省临床医学中心建设项目资助(消化疾病中心);海南省卫生健康行业科研项目(22A200264)。

摘　　要：目的探讨通关藤对胃癌小鼠类固醇受体共激活因子(Steroid receptor coactivator,Src)/黏着斑激酶(Focaladhesion kinase,FAK)信号通路及Th1/Th2相关因子水平的影响。方法将SPF级成年雌雄各半C57BL/6小鼠60只,根据随机数字表法分为空白对照组、模型组(注射生理盐水)、环磷酰胺组(注射环磷酰胺)、通关藤低、中、高剂量组(分别注射0.1 ml、0.2 ml、0.4 ml通关藤注射液),每组各10只。干预1次/2 d,于干预第21天时处死所有小鼠,颈部取血。测量小鼠体质量以及胸腺、脾、肿瘤质量。HE染色观察小鼠瘤体病理形态变化。流式细胞术检测小鼠CD4^(+)、CD8^(+)以及CD4^(+)/CD8^(+)水平,ELISA法检测白细胞介素-2(Interleukin-2,IL-2)、白细胞介素-4(Interleukin-4,IL-4)、白细胞介素-10(Interleukin-10,IL-10)、干扰素-γ(Interferon-γ,INF-γ)水平,Western blot法检测Src、磷酸化Src(p-Src)、FAK、磷酸化FAK(p-FAK)蛋白表达水平。结果与模型组比较,通关藤各干预组脾指数、胸腺指数水平升高,且随通关藤剂量增加,脾指数、胸腺指数水平、抑瘤率均升高,差异有统计学意义(P<0.05)。环磷酰胺组脾指数和胸腺指数明显低于各通关藤剂量组,差异有统计学意义(P<0.05);环磷酰胺组和通关藤高剂量组抑瘤率明显高于通关藤低、中剂量组,差异有统计学意义(P<0.05)。HE染色结果显示,模型组肿瘤细胞排列整齐且密集,而环磷酰胺组和通关藤各剂量组肿瘤细胞密度有所减少,且分布不均,并呈不同程度的肿瘤细胞坏死灶。与模型组比较,通关藤各干预组IL-2、INF-γ水平升高,IL-4、IL-10水平降低,差异有统计学意义(P<0.05);相较于环磷酰胺组,通关藤低、中、高剂量组IL-2、INF-γ水平升高,IL-4、IL-10水平降低,差异有统计学意义(P<0.05),且通关藤干预剂量越高,IL-2、INF-γ水平升高越明显,IL-4、IL-10水平降低越明显(P<0.05)。与模型组比较,环磷酰胺组和通关藤各干预组Objective To investigate the effects of Marsdeniae Tenacissimae Caulis on steroid receptor coactivator(Src)/focal adhesion kinase(FAK)signaling pathway and Th1/Th2-related cytokine levels in a mouse model of gastric cancer.Methods A total of 60 adult SPF-grade C57BL/6 mice,equally divided between males and females,were randomly assigned to six groups using the random number table method:blank control group,model group(injected with saline),cyclophosphamide group(injected with cyclophosphamide),and three Marsdeniae Tenacissimae Caulis groups with low,medium,and high doses(injected with 0.1,0.2 and 0.4 mL,of Marsdeniae Tenacissimae Caulis extract,respectively),with 10 mice in each group.Interventions were administered once every 2 days.On the 21st day,all mice were sacrificed,and blood samples were collected from the neck.The body weight,thymus weight,spleen weight,and tumor weight of the mice were measured.HE staining was used to observe the pathological changes in the tumor tissue.Flow cytometry was used to detect the levels of CD4^(+),CD8^(+),and the CD4^(+)/CD8^(+)ratio.ELISA was used to measure the levels of interleukin-2(IL-2),interleukin-4(IL-4),interleukin-10(IL-10),and interferon-γ(INF-γ).Western blot analysis was used to detect the expression levels of Src,phosphorylated Src(p-Src),FAK,and phosphorylated FAK(p-FAK).Results Compared with the model group,the spleen and thymus indices in the Marsdeniae Tenacissimae Caulis intervention groups increased,and these indices,along with the tumor inhibition rate,increased with the dosage of Marsdeniae Tenacissimae Caulis,with statistically significant differences(P<0.05).The spleen and thymus indices in the cyclophosphamide group were significantly lower than those in the Marsdeniae Tenacissimae Caulis groups,with statistically significant differences(P<0.05).The tumor inhibition rates in the cyclophosphamide and high-dose Marsdeniae Tenacissimae Caulis groups were significantly higher than those in the low-and medium-dose Marsdeniae Tenacissimae Caulis groups,wi

关 键 词：通关藤 胃癌 类固醇受体共激活因子 黏着斑激酶 Th1/Th2 

分 类 号：R285.5[医药卫生—中药学]