Regulatory Mechanisms for Transcriptional Bursting Revealed by an Event-Based Model  

作　　者：Renje Wu Bangyan Zhou Wei Wang Feng Liu 

机构地区：[1]National Laboratory of Solid State Microstructures,Department of Physics,and Collaborative Innovation Center of Advanced Microstructures,Nanjing University,Nanjing 210093,P.R.China [2]Institute for Brain Sciences,Nanjing University,Nanjing 210093,P.R.China.

出　　处：《Research》2024年第3期217-231,共15页研究（英文）

基　　金：the National Natural Science Foundation of China(11874209 and 12090052).

摘　　要：Gene transcription often occurs in discrete bursts, and it can be difficult to deduce the underlying regulatory mechanisms for transcriptional bursting with limited experimental data. Here, we categorize numerous states of single eukaryotic genes and identify 6 essential transcriptional events, each comprising a series of state transitions;transcriptional bursting is characterized as a sequence of 4 events, capable of being organized in various configurations, in addition to the beginning and ending events. By associating transcriptional kinetics with mean durations and recurrence probabilities of the events, we unravel how transcriptional bursting is modulated by various regulators including transcription factors. Through analytical derivation and numerical simulation, this study reveals key state transitions contributing to transcriptional sensitivity and specificity, typical characteristics of burst profiles, global constraints on intrinsic transcriptional noise, major regulatory modes in individual genes and across the genome, and requirements for fast gene induction upon stimulation. It is illustrated how biochemical reactions on different time scales are modulated to separately shape the durations and ordering of the events. Our results suggest that transcriptional patterns are essentially controlled by a shared set of transcriptional events occurring under specific promoter architectures and regulatory modes, the number of which is actually limited.

关 键 词：KINETICS essentially CONSTRAINTS 

分 类 号：P20[天文地球—测绘科学与技术]