A FRET-Based Ratiometric H_(2)S Sensor for Sensitive Optical Molecular Imaging in Second Near-Infrared Window  

作　　者：Shan Lei Kejia Jiang Chenqing Zhang Wei Sun Yuantao Pan Dong Wang Peng Huang Jing Lin 

机构地区：[1]Marshall Laboratory of Biomedical Engineering,International Cancer Center,Shenzhen Key Laboratory of Tumor Visualization Molecular Medicine,Laboratory of Evolutionary Theranostics(LET),School of Biomedical Engineering,Shenzhen University Medical School,Shenzhen University,Shenzhen 518055,China. [2]Center for AIE Research,College of Materials Science and Engineering,Shenzhen University,Shenzhen 518060,China.

出　　处：《Research》2024年第3期671-684,共14页研究（英文）

基　　金：the National Key R&D Program of China(2018YFA0704000);the National Natural Science Foundation of China(82302362,82071985,and 82372116);the Basic Research Program of Guangdong(2022A1515010620);the Basic Research Program of Shenzhen(JCYJ20200109105620482,JCYJ20220818095806014,and KQTD20190929172538530).

摘　　要：Second near-infrared(NIR-II)window optical molecular imaging kicks off a new revolution in high-quality imaging in vivo,but always suffers from the hurdles of inevitable tissue autofluorescence background and NIR-II probe development.Here,we prepare a Förster resonance energy transfer-based ratiometric NIR-II window hydrogen sulfide(H_(2)S)sensor through the combination of an H_(2)S-responsive NIR-II cyanine dye(acceptor,LET-1055)and an H_(2)S-inert rhodamine hybrid polymethine dye(donor,Rh930).This sensor not only exhibits high sensitivity and selectivity,but also shows rapid reaction kinetics(~20 min)and relatively low limit of detection(~96 nM)toward H_(2)S,allowing in vivo ratiometric NIR-II fluorescence imaging of orthotopic liver and colon tumors and visualization of the drug-induced hepatic H_(2)S fluctuations.Our findings provide the potential for advancing the feasibility of NIR-II activity-based sensing for in vivo clinical diagnosis.

关 键 词：H_ Infrared kinetics 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]