4种微生物杀虫剂对点尾尺蛾幼虫的时间-剂量-死亡率模型分析  

Time-dose-mortality model of four microbial insecticides on Ourapteryx nigrociliaris Leech

作　　者：王姝婕 刘子业 孙斌 卢小军 温俊宝[1] WANG Shujie;LIU Ziye;SUN Bin;LU Xiaojun;WEN Junbao(State Key Laboratory of Efficient Production of Forest Resources,Beijing Key Laboratory for Forest Pest Control,Beijing Forestry University,Beijing 100083,China;Dongcha Forest Farm of Xiaolong Mountain Forest Conservation Center,Gansu Province,Tianshui 741035,China)

机构地区：[1]林木资源高效生产全国重点实验室,林木有害生物防治北京市重点实验室,北京林业大学,北京100083 [2]甘肃省小陇山林业保护中心东岔林场,天水741035

出　　处：《植物保护》2024年第5期129-136,共8页Plant Protection

基　　金：国家自然科学基金(32071774)。

摘　　要：为筛选适用于防治点尾尺蛾Ourapteryx nigrociliaris的微生物杀虫剂,发展粗榧Cephalotaxus sinensis等药用植物害虫的生物防治,采用浸渍法测定了甘蓝夜蛾核型多角体病毒Mamestra brassicae nuclear polyhedrosis virus、苏云金芽胞杆菌Bacillus thuringiensis、球孢白僵菌Beauveria bassiana、金龟子绿僵菌Metarhizium anisopliae CQMa421对点尾尺蛾幼虫的致死效应,并使用时间-剂量-死亡率(TDM)模型进行模拟。结果表明,TDM模型均通过Hosmer-Lemoshow拟合异质性检验,4种微生物杀虫剂处理10 d的幼虫校正死亡率最高分别达到79.31%、86.21%、93.10%和72.41%,半致死浓度LC_(50)分别为1.81×10^(6)PIB/mL、2.71×10^(3)IU/mL、9.40×10^(6)孢子/mL以及9.86×10^(6)孢子/mL。综合考虑药剂致死率和致死速度,球孢白僵菌、苏云金芽胞杆菌和甘蓝夜蛾核型多角体病毒的杀虫效果优于金龟子绿僵菌CQMa421,更具应用潜力。本研究为点尾尺蛾的生物防治提供了参考依据。To identify microbial pesticides suitable for controlling Ourapteryx nigrociliaris and develop biocontrol strategies for pests damaging Cephalotaxus sinensis and other medicinal plants,the laboratory toxicities of Mamestra brassicae nuclear polyhedrosis virus,Bacillus thuringiensis,Beauveria bassiana,and Metarhizium anisopliae strain CQMa421 against O.nigrociliaris larvae were measured by using the dipping method.A time-dose-mortality model(TDM)was established and validated using the Hosmer-Lemeshow test.The highest corrected mortality rates of the four microbial pesticides reached 79.31%,86.21%,93.10%,and 72.41%,respectively.The LC_(50)values were determine as 1.81×10^(6)PIB/mL,2.71×10^(3)IU/mL,9.40×10^(6)spores/mL,and 9.86×10^(6)spores/mL,respectively.Considering both mortality rate and speed of mortality,B.bassiana,B.thuringiensis,and Mamestra brassicae nuclear polyhedrosis virus demonstrated superior insecticidal efficacy compared to M.anisopliae strain CQMa421,demonstrating higher potential for application.This study provides valuable insights for the biocontrol of O.nigrociliaris.

关 键 词：点尾尺蛾 粗榧 微生物杀虫剂 时间剂量死亡率模型 毒力测定 

分 类 号：S763.7[农业科学—森林保护学]