4种方法脱除太子参BBWV和TuMV病毒的效果比较  

Comparision of effect of four methods for the virus-free of BBWV and TuMV viruses in Pseudostellaria heterophylla

作　　者：步洪松 王丁 张馨月 周丹 郑生文 匡云波 叶祖云 BU Hongsong;WANG Ding;ZHANG Xinyue;ZHOU Dan;ZHENG Shengwen;KUANG Yunbo;YE Zuyun(College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;The Engineering Technology Research Center of Characteristic Medicinal Plants of Fujian,College of Life Sciences,Ningde Normal University,Ningde 352100,China;Fujian Zheshen Biotechnology Research Co.,Ltd.,Ningde 355300,China)

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]福建省特色药用植物工程技术研究中心,宁德师范学院生命科学学院,宁德352100 [3]福建柘参生物科技研究股份有限公司,宁德355300

出　　处：《植物保护》2024年第5期213-218,共6页Plant Protection

基　　金：中央引导地方科技发展专项资金项目(202113030);宁德师范学院科研项目(2020FZ07,2021FZ03,2022ZX01);福建省特色药用植物工程技术研究中心学生科研项目(PC202101,PC202104)。

摘　　要：以太子参‘柘参1号’品种为试验材料,比较微茎尖培养、低温处理(2~4℃)结合微茎尖培养、超低温(-196℃)培养、种胚培养等4种方法对蚕豆萎蔫病毒(broad bean wilt virus,BBWV)和芜菁花叶病毒(turnip mosaic virus,TuMV)的脱除效果,结果显示采用低温处理结合微茎尖培养,太子参成活率为64.7%,BBWV和TuMV的脱毒率分别为63.6%和31.8%;采用种胚培养的成活率为56.7%,对BBWV和TuMV的脱毒率均可达100%。病毒TuMV相较BBWV更难脱除。The effects of four methods,including microstem tip culture,low temperature(2-4℃)treatment combined with microstem tip culture,ultra-low temperature(-196℃)culture and embryo culture,on the virus-free of broad bean wilt virus(BBWV)and turnip mosaic virus(TuMV)from Pseudostellaria heterophylla‘Zheshen No.1’were compared.The results showed that the survival rate of P.heterophylla was 64.7%,and the virus-free rates of BBWV and TuMV were 63.6%and 31.8%,respectively,using low temperature treatment combined with micro-stem tip culture.The survival rate of embryo culture was 56.7%,and the virus-free rate of BBWV and TuMV was 100%.P.heterophylla mosaic virus TuMV is more difficult to remove than BBWV.

关 键 词：太子参 花叶病毒 脱病毒方法 病毒检测 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]