黄芪多糖调控Bcl-2/Bax信号通路抑制卵巢腺癌Caov-3细胞生长的实验研究  

Astragalus Polysaccharides Inhibit the Growth of Ovarian Adenocarcinoma Caov-3 Cells by Regulating the Bcl-2/Bax Signaling Pathway:An Experimental Study

作　　者：张永跟[1] 颜小飞 刘锋 贾学昭 蔡玥 刘莹[1] 李玲秀[1] 李学军[2] ZHANG Yonggen;YAN Xiaofei;LIU Feng;JIA Xuezhao;CAI Yue;LIU Ying;LI Lingxiu;LI Xuejun(Anhui University of Chinese Medicine,Anhui Hefei 230012,China;The Second Affiliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230061,China)

机构地区：[1]安徽中医药大学,安徽合肥230012 [2]安徽中医药大学第二附属医院,安徽合肥230061

出　　处：《安徽中医药大学学报》2024年第5期54-58,共5页Journal of Anhui University of Chinese Medicine

基　　金：安徽省高等学校省级质量工程项目(2020jyxm1017);安徽中医药大学校级纵向探索课题(2021zxts42);安徽省自然科学基金项目(2008085MH265)。

摘　　要：目的研究黄芪多糖调控Bcl-2/Bax信号通路对卵巢腺癌Caov-3细胞生长的影响。方法将黄芪多糖分为低、中、高剂量组,分别加入10、50、100 mg/mL黄芪多糖,流式细胞术检测10、50、100 mg/mL黄芪多糖作用Caov-3细胞后对细胞周期的影响,显微镜下观察Caov-3细胞凋亡情况和形态变化,Western blot法检测3组药物作用Caov-3细胞后对Bcl-2、Bax蛋白表达水平及对Bcl/Bax的影响;同时,比较阿司匹林联合黄芪多糖与单用黄芪多糖对Caov-3细胞的抑制率。结果黄芪多糖低、中、高剂量组对Caov-3细胞的抑制率分别为40%、50%、65%,黄芪多糖的IC_(50)为50 mg/mL,与对照组比较,黄芪多糖高剂量组对Caov-3细胞的抑制效果最为明显,差异具有统计学意义(P<0.05);黄芪多糖联合阿司匹林的抑制效果优于单纯使用黄芪多糖(P<0.05)。与对照组比较,黄芪多糖高剂量组对Caov-3细胞周期的影响更为显著,差异具有统计学意义(P<0.05)。与对照组比较,黄芪多糖高剂量组Caov-3细胞凋亡的数目显著增加,Bcl-2蛋白表达水平显著降低,Bax蛋白表达水平显著升高,Bcl-2/Bax降低,差异均有统计学意义(P<0.05)。结论黄芪多糖能有效抑制Caov-3细胞的生长,联合阿司匹林效果更好。其机制可能与黄芪多糖调控Bcl-2/Bax信号通路,促进细胞凋亡有关。Objective To investigate the effect of Astragalus polysaccharides on the growth of ovarian adenocarcinoma Caov-3 cells by regulating the Bcl-2/Bax signaling pathway.Methods Caov-3 cells were divided into low-,middle-,and high-dose groups and were added with Astragalus polysaccharides at a concentration of 10,50,and 100 mg/mL,respectively.Flow cytometry was used to observe the effect on cell cycle after Caov-3 cells were treated with Astragalus polysaccharides at a concentration of 10,50,and 100 mg/mL,respectively;microscopy was performed to observe the apoptosis and morphological changes of Caov-3 cells;Western blot was used to observe the effect of Astragalus polysaccharides on the protein expression levels of Bcl-2 and Bax and Bcl-2/Bax ratio.Meanwhile,the inhibition rate of Caov-3 cells was compared between aspirin combined with Astragalus polysaccharides and Astragalus polysaccharides used alone.Results The inhibition rates of Caov-3 cells in the low-,middle-,and high-dose Astragalus polysaccharides groups were 40%,50%,and 65%,respectively,and the half-maximal inhibitory concentration of Astragalus polysaccharides was 50 mg/mL.Compared with the control group,the high-dose Astragalus polysaccharides group had the most significant inhibitory effect on Caov-3 cells(P<0.05),and Astragalus polysaccharides combined with aspirin had a significantly better inhibitory effect than Astragalus polysaccharides alone(P<0.05).Compared with the control group,the high-dose Astragalus polysaccharides group had a significantly greater effect on the cell cycle of Caov-3 cells(P<0.05).Compared with the control group,the high-dose Astragalus polysaccharides group had a significant increase in the number of apoptotic Caov-3 cells,a significant reduction in the protein expression level of Bcl-2 and Bcl-2/Bax ratio,and significant increases in the protein expression level of Bax(P<0.05).Conclusion Astragalus polysaccharides can effectively inhibit the growth of Caov-3 cells and has a better effect when combined with aspirin,possibly

关 键 词：黄芪多糖 Bcl-2/Bax信号通路 卵巢腺癌Caov-3细胞 细胞凋亡 

分 类 号：R285[医药卫生—中药学]