响应面法优化柞蚕蚕沙总黄酮提取工艺及体外抗氧化活性分析  

Optimization of Total Flavonoids Extraction Process and in Vitro Antioxidant Activity of Tussah’s Excrement by Response Surface Methodology

作　　者：张春杨 张月 王大雨 周影 钱琨 杨淑芳[2] 朴玉兰 ZHANG Chunyang;ZHANG Yue;WANG Dayu;ZHOU Ying;QIAN Kun;YANG Shufang;PIAO Yulan(Jilin Agricultural Science and Technology University,Jilin Jilin 132012,China;Jilin Province Sericultural Research Institute,Jilin Jilin 132109,China)

机构地区：[1]吉林农业科技学院,吉林吉林132109 [2]吉林省蚕业科学研究院,吉林吉林132012

出　　处：《北方蚕业》2024年第3期23-28,37,共7页North Sericulture

基　　金：吉林省教育厅科学技术研究项目(JJKH20230439KJ)。

摘　　要：目的:优化柞蚕蚕沙总黄酮提取工艺,并考察其体外抗氧化活性。方法:以黄酮得率为评价指标,考察提取过程中乙醇体积分数、提取温度、料液比和超声功率对得率的影响。在单因素试验结果的基础上,采用Box-behnken中心组合设计原理优化工艺条件。以DPPH、ABTS法测定所得总黄酮的抗氧化能力。结果:最优提取工艺为乙醇体积分数62%,提取温度64℃,料液比1:23,超声功率384 w,总黄酮得率为3.6%。柞蚕蚕沙总黄酮对DPPH自由基清除率和ABTS自由基清除率的IC50分别为0.405 mg/mL、0.115 mg/mL。结论:优化后的提取工艺稳定可靠,所得柞蚕蚕沙总黄酮具有良好的抗氧化活性。To optimize the extraction process of total flavonoids from tussah’s excrement and to investigate the in vitro antioxidant activities,the effects of ethanol volume fraction,extraction temperature,material-liquid ratio,and ultrasonic power on the yield of flavonoids during the extraction process were investigated using the yield of flavonoids as the evaluation index.Based on the results of the one-way test,the Box-behnken central combination design principle was used to optimize the process conditions.The antioxidant capacity of total flavonoids was determined by DPPH and ABTS methods.The optimal extraction process was an ethanol volume fraction of 62%,an extraction temperature of 64℃,a material-liquid ratio of 1:23,and an ultrasonic power of 384 w,resulting in a total flavonoid yield of 3.6%.The IC50 of the total flavonoids from the tussah’s excrement against DPPH and ABTS free radical scavenging were found to be 0.405 mg/mL and 0.115 mg/mL,respectively.The optimized extraction process is stable and reliable,and the total flavonoids obtained from tussah’s excrement exhibit good antioxidant activity.

关 键 词：柞蚕蚕沙 黄酮 Box-behnken响应面法 DPPH ABTS 抗氧化 

分 类 号：R284.2[医药卫生—中药学]