盐胁迫下不同野生草莓品种生理响应及耐盐性差异分析  

Physiological Responses of Different Wild Strawberry Varieties Under Salt Stress and Differential Analysis of Salt Tolerance

作　　者：江毅 李晨 刘凡凡 王晓宇 杨静静 薛浩 JIANG Yi;LI Chen;LIU Fanfan;WANG Xiaoyu;YANG Jingjing;XUE Hao(College of Horticulture,Anhui Agricultural University,Hefei,Anhui 230036)

机构地区：[1]安徽农业大学园艺学院,安徽合肥230036

出　　处：《北方园艺》2024年第18期32-40,共9页Northern Horticulture

基　　金：安徽省自然科学基金青年资助项目(2108085QC120);安徽农业大学校青年基金资助项目(2019zd25);2022年省级大创资助项目(S202210364240)。

摘　　要：以5种二倍体野生草莓为试材,采用不同浓度盐胁迫处理的方法,测定了叶片相对含水量(RWC)、叶绿素含量(SPAD)、丙二醛(MDA)、活性氧(O_(2)^(-·)和H_(2)O_(2))、超氧化物歧化酶(SOD)和过氧化物酶(POD)等7个生理指标,采用主成分分析法研究其耐盐性差异,以期为筛选出耐盐性强的野生草莓品种提供参考依据。结果表明:盐胁迫下5种野生草莓表现出不同的盐害级别,其相对含水量和叶绿素含量呈下降趋势,丙二醛和活性氧含量均呈上升趋势,超氧化物歧化酶和过氧化物酶等抗氧化酶活性基本呈先上升后下降的趋势。通过主成分分析得出不同野生草莓品种的耐盐性强弱,即‘15-5’>‘02-9森林’>‘05-12’>‘Ruegen’>‘44-6’。Taking five diploid wild strawberry cultivars as the test materials,seven physiological indexes of salt resistance,included relative water content(RWC),chlorophyll content(SPAD),malondialdehyde content(MDA),ROS content(O_(2)^(·-) and H_(2)O_(2)),superoxide dismutase(SOD)and peroxidase(POD),were measured and analyzed in seedling stage with different levels of salt stress treatments.Then the salt tolerances of five strawberry cultivars were evaluated based on principal components analysis,in order to provide reference for screening wild strawberry varieties with strong salt tolerance.The results showed that the RWC and SPAD value show decreasing trend,MDA and ROS contents increased,the activity of antioxidant enzymes(SOD and POD)increased first and then decreased with the increase of salt stress time.But there were large differences of variations in seven physiological indexes of different cultivars.Through the principal component analysis of salt stress treatment,the salt tolerance of five wild strawberry cultivars was obtained,which was‘15-5’>‘02-9 Forest’>‘05-12’>‘Ruegen’>‘44-6’.

关 键 词：野生草莓 盐胁迫 生理响应 差异分析 

分 类 号：S668.4[农业科学—果树学]