栝楼病果微生物多样性分析及病原菌的分离鉴定  

Microbial Diversity Analysis of Trichosanthes kirilowii Maxim.Fruits and Identification of Pathogenic Pathomycete

作　　者：邢铃凯 曹舜 李卫文[3] 程有余 赵伟 谢冬梅 XING Lingkai;CAO Shun;LI Weiwen;CHENG Youyu;ZHAO Wei;XIE Dongmei(College of Pharmacy,Anhui University of Chinese Medicine,Hefei,Anhui 230012;Institute of Plant Protection and Agro-products Safety,Anhui Academy of Agricultural Sciences,Hefei,Anhui 230031;Institute of Horticulture,Anhui Academy of Agricultural Sciences,Hefei,Anhui 230031;Anhui You Yu Kua Yue Gua Lou Food Development Co.Ltd.,Qianshan,Anhui 246300)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安徽省农业科学院植物保护与农产品质量安全研究所,安徽合肥230031 [3]安徽省农业科学院园艺研究所,安徽合肥230031 [4]安徽有余跨越瓜蒌食品开发有限公司,安徽潜山246300

出　　处：《北方园艺》2024年第18期100-107,共8页Northern Horticulture

基　　金：安徽中医药大学-潜山市医药健康发展技术研究中心科研攻关资助项目(2021QSHZ01-2)。

摘　　要：以栝楼健康果和病果为试材,采用高通量测序技术与分子鉴定的方法,研究栝楼病果与健康果表面微生物多样性差异,探究引起栝楼果实致病菌的种类,以期为栝楼病害的科学防治提供参考依据。结果表明:从所有样本中共得到8个门、24个纲、54个目、133个科、206个属、286个种,其中属水平排列前5的依次为间座壳菌属(Diaporthe)、枝孢菌属(Cladosporium)、蔓枯菌属(Stagonosporopsis)、炭疽菌属(Colletotrichum)、酵母菌属(Hannaella)。患病果实表面微生物的集中度高,与健康组微生物群落结构具有明显差异,其中丰度较高微生物主要为间座壳属(Diaporthe)、炭疽菌属(Colletotrichum)真菌;选择4株分离纯化后的典型菌株进行DNA分子鉴定,结果显示为炭疽属(Colletotrichum)、间座壳属(Diaporthe)真菌,验证了微生物多样性分析结果。综合分析可知,引起潜山市栝楼果实病害的病原菌为间座壳属(Diaporthe)、炭疽菌属(Colletotrichum)真菌。Taking healthy and diseased fruits of Trichosanthes kirilowii as the test material,next generation sequencing(NGS)technology and molecular identification method were used to analyze the healthy and diseased fruits to explore the species of pathomycete of T.kirilowii fruits,in order to provide reference for scientific prevention and control of diseases in Trichosanthes kirilowii.The results showed that a total of 8 phyla,24 orders,54 classes,133 families,206 genera,and 286 species were obtained from all samples.Among them,the top five genera were Diaporthe,Cladosporium,Stagonosporopsis,Colletotrichum and Hannaella.There was a high concentration of microorganisms on the diseased fruits,and the microbial community structure was significantly different from the healthy group.The microorganisms with high abundance were mainly Diaporthe and Colletotrichum.The fungus of Trichosanthes kirilowii Maxim.were isolated and purified.Four typical strains were selected for DNA molecular identification.The results showed that the fungi were Colletotrichum,Diaporthe,which verified the results of microbial diversity analysis.In summary,Diaporthe and Colletotrichum fungi were identified as the pathomycete causing the disease of T.kirilowii in Qianshan city.

关 键 词：栝楼 病原菌 高通量测序 分子鉴定 

分 类 号：S567.9[农业科学—中草药栽培]