蔷薇科植物越冬病残体中梨火疫病菌的检测及致病性分析  

Detection and pathogenicity analysis of Erwinia amylovora bacteria in overwintering plant debris of rosaceae plants

作　　者：王杰花 王岩 和子森 韩丽丽[4] 陈卫民[4] 吴品珊[5] 雷荣[5] WANG Jiehua;WANG Yan;HE Zisen;HAN Lili;CHEN Weimin;WU Pinshan;LEI Rong(Xinjiang Yili Prefecture Institute of Agricultural Sciences,Yining 835000;Xinjiang Academy of Forestry;Forestry Pest Control and Quarantine Bureau of Xinjiang Uygur Autonomous Region;Yili Vocational and Technical College;Chinese Academy of Inspection and Quarantine)

机构地区：[1]新疆伊犁州农业科学研究所,伊宁835000 [2]新疆林业科学院 [3]新疆维吾尔自治区林业有害生物防治检疫局 [4]伊犁职业技术学院 [5]中国检验检疫科学研究院

出　　处：《中国果树》2024年第10期107-112,共6页China Fruits

基　　金：国家重点研发计划(2021YFC2600400);伊犁州外来入侵有害生物监测与防控重点实验室;伊犁州重点研究与技术开发项目(YZ2022B011);伊犁州应用基础研究项目(YJC2023A14)。

摘　　要：为明确梨火疫病在新疆地区发生、流行的初侵染来源,以新疆巴州库尔勒市、轮台县,伊犁地区霍城县、伊宁市采集的6种蔷薇科植物越冬病残体为试材,采用平板分离划线培养、致病性测试、PCR检测和序列分析等方法,检测病残体是否携带梨火疫病菌(Erwinia amylovora)及测定存活力。结果表明,6种寄主的病残体组织均可分离出病原菌,其分离出的病原菌接种在离体叶片上均可致病,通过分子生物学鉴定为梨火疫病菌。6种寄主的病残体(花腐、叶枯、枝枯、果腐、溃疡斑组织)均携带梨火疫病菌,且具有致病性,因此,需及时清除果园中越冬病残体。研究结果为防控梨火疫病的发生和流行提供了理论依据。In order to determine the origin of the initial infection of pear fire blight in Xinjiang.The overwintering disease residues of 6 species of rosae were collected from Korla City and Luntai County of Bazhou,Huocheng County and Yining City of Yili Prefecture.Using the methods of plate isolation and streak culture,pathogenicity test,PCR detection and sequence analysis,the existence and activity of pear fire blight in the diseased remains were studied.The results showed that the pathogenic bacteria could be isolated from the diseased tissues of the 6 host species,and the isolated pathogens could be pathogenic when inoculated on the isolated leaves,and were identified as pear fire blight by molecular biology.Erwinia amylovora was found to be pathogenic in the remnants of 6 host species(flower rot,leaf rot,branch rot,fruit rot,ulcerative plaque tissue).Therefore,it provides a theoretical basis for timely removal of overwintering disease residues in orchards and prevention and control of the occurrence and prevalence of pear fire blight.

关 键 词：蔷薇科 病残体 梨火疫病菌 检测 致病性 

分 类 号：S436.6[农业科学—农业昆虫与害虫防治]