喷施纳米TiO_(2)对青菜生长及土壤细菌群落的影响  

Effects of TiO_(2) nanoparticles on green vegetable(Brassica chinensis L.)growth and soil bacterial community

作　　者：罗静仪 徐江兵 余振 刘蕾[1] 周国逸 LUO Jingyi;XU Jiangbing;YU Zhen;LIU Lei;ZHOU Guoyi(School of Ecology and Applied Meteorology,Nanjing University of Information Science&Technology,Nanjing 210044,China)

机构地区：[1]南京信息工程大学生态与应用气象学院,南京210044

出　　处：《应用与环境生物学报》2024年第4期680-685,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(42130506)资助。

摘　　要：为了解纳米TiO_(2)的植物促长机理及微生物的反馈作用采用,温室盆栽实验,探究喷施不同浓度纳米TiO_(2)(0、50、100、150 mg/L,分别用CK、L、M、H表示)对青菜生物量及叶片光合特性的影响,并采用高通量测序分析青菜根际细菌群落多样性和组成的变化.结果表明,喷施纳米TiO_(2)能增加青菜生物量、叶片叶绿素a含量、叶绿素b含量、净光合速率,其中M处理影响最显著,与CK相比增幅分别为25.51%、20.29%、23.33%和30.86%.青菜根际土壤细菌群落分析表明,喷施纳米TiO_(2)对土壤细菌群落alpha多样性指数无显著影响,但改变了细菌群落的组成,其中M处理的群落结构与CK处理相似度较低,显著增加了芽孢杆菌(Bacillales)和芽孢单胞菌(Gemmatimonadales)的相对丰度,但显著降低了红古菌(Solibacterales)和硫单胞菌(Desulfuromonadales)的相对丰度.进一步通过冗余分析(RDA)发现,M处理与青菜的生物量、叶绿素含量和光合速率呈正相关.相关性分析表明,着色菌(Chromatiales)和Gemmatimonadales等类群的相对丰度与植物生长指标呈显著正相关,硝化螺旋菌(Nitrospirales)与叶绿素的含量呈显著负相关,Solibacterales、Desulfuromonadales与青菜生物量以及净光合速率呈显著负相关.因此,喷施适宜浓度的纳米TiO_(2)可促进青菜生长,增加叶片叶绿素含量,进而改变土壤细菌群落组成;上述研究结果可为理解纳米TiO_(2)在植物-土壤系统中的作用机制及在农业生产中的应用提供理论基础和参考依据.(图3表2参28)To understand the mechanisms by which nano TiO_(2) promotes plant growth and feedback effects on microorganisms and investigate the changes in microbial communities induced by nano TiO_(2) treatment,a pot experiment was conducted under greenhouse conditions by spraying different concentrations of TiO_(2) nanoparticles(0,50,100,and 150 mg/L;denoted as CK,L,M,and H,respectively)onto the surfaces of Chinese cabbage leaves.Plant biomass and photosynthetic characteristics of plant leaves were measured.Highthroughput sequencing was employed to analyze changes in the diversity and composition of the rhizosphere bacterial community.The results showed that spraying with TiO_(2) nanoparticles promoted the growth of Chinese cabbage.It notably increased plant biomass,chlorophyll-a and chlorophyll-b content,and the net photosynthetic rate of plant leaves.The most significant increases were observed in the M treatment,with those values 25.51%,20.29%,23.33%,and 30.86% higher than those in CK,respectively.High-throughput sequencing showed no significant changes in the alpha diversity of the rhizosphere bacterial community.However,the composition and structure of the bacterial community were significantly shifted by the M treatment.Specifically,the M treatment significantly increased the relative abundances of Bacillales and Gemmatimonadales,but decreased those of Solibacterales and Desulfuromonadales.Such features led to a profound dissimilarity in bacterial community structure under the M treatment compared to CK.Furthermore,redundancy analysis(RDA)showed positive correlations between the bacterial community and Chinese cabbage biomass,chlorophyll content,and photosynthetic rate in the M treatment.Correlation analysis showed that bacterial lineages such as Chromatiales and Gemmatimonadales were positively correlated with plant growth parameters,Nitrospirales was significantly and negatively correlated with chlorophyll content,and Soilbacterales and Desulfuromonadales were significantly and negatively correlated with plant bioma

关 键 词：纳米TiO_(2) 净光合速率 青菜生长 土壤微生物 细菌群落结构 

分 类 号：S634.3[农业科学—蔬菜学] S154.3[农业科学—园艺学]