盐胁迫下接种内生真菌对短枝木麻黄幼苗生长的影响  

Effect of endophytic fungi inoculation on the growth of Casuarina equisetifolia seedlings under salt stress

作　　者：李冠军 余雯静 温昕怡 苏亲桂 吴承祯[1,2] 苏军 李键[1,2] LI Guanjun;YU Wenjing;WEN Xinyi;SU Qingui;WU Chengzhen;SU Jun;LI Jian(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Higher Educational Key Laboratory for Forest Ecosystem Process and Management of Fujian Province,Fuzhou 350002,China;Fujian Chihu State-owned Forest Shelter Farm,Huian 362100,China)

机构地区：[1]福建农林大学林学院,福州350002 [2]森林生态系统过程与经营福建省高校重点实验室,福州350002 [3]福建省惠安赤湖国有防护林场,福建惠安362100

出　　处：《应用与环境生物学报》2024年第4期777-785,共9页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(32071753)资助。

摘　　要：为明晰盐分胁迫下内生真菌对宿主根系生长的影响,将一年生短枝木麻黄(Casuarina equisetifolia)幼苗栽植到不同盐度(质量分数为0、3‰、6‰、9‰)土壤中,以添加菌株培养基(CK)为对照,研究接种耐盐性菌株葡萄座腔菌Botryosphaeria Z1(Z1)、炭团菌Hypoxylon Y6(Y6)及其混合菌剂(Z1+Y6)对其宿主幼苗根系形态与活力、养分吸收以及苗高、地径和根冠比等的影响.结果表明:土壤盐胁迫下,木麻黄幼苗根系活力、根系总长(TRL)、总根表面积(TRSA)、总根体积(TRV)、总根直径(TRD)、根长密度(RLD)、根表面积密度(SAD)降低,根组织密度(RTD)增加;接菌处理显著提高了根系活力和生物量,同一菌株处理下根系形态和养分特征受土壤盐度变化影响较大;与CK相比,接菌处理显著提高了TRD以及3‰和6‰盐度下根系活力、全磷(TP)含量和9‰盐度下TRV、TRSA和RSD.此外,3‰盐度下,Z1菌处理的TRL、RLD、根全氮(TN)含量显著高于CK,碳磷比(C/P)显著低于CK.Z1+Y6菌处理还显著提高了3‰和6‰盐度下TRSA、TRV、SAD和根碳磷比(C/P).9‰盐度下,Z1和Z1+Y6菌处理下TP含量和根系活力同样显著高于CK.采用逐步回归建立了回归方程模型,发现RLD是影响苗高的主要因素,根系活力和TC含量是影响地径的主要因素;通径分析表明RTD、TRV、TP和TRD为影响生物量的主要因素,其中TRD的直接作用影响最大,为正向贡献.综上,盐胁迫下接菌处理能够影响幼苗根系形态和养分吸收以及生物量积累,耐盐性菌株可能是通过诱导宿主根系生长发育提高自身耐盐性,其中混合菌剂更有利于缓解盐害;结果可为深入开发木麻黄抗逆微生物制剂提供科学依据.(图3表3参57)The aim of this study was to investigate the effects of adding two salt-tolerant endophytic fungi and their mixtures on root morphology,root activity of nutrient elements,seedling height,ground diameter,and biomass of Casuarina seedlings under soil stress with different salinities.Casuarina equisetifolia seedlings were planted in soils with different salinities(mass fractions:0‰,3‰,6‰,and 9‰).Botryosphaeria Z1(Z1),Hypoxylon Y6(Y6),and their mixture(Z1+Y6)were used as treatments,and the carrier(CK)was used as the control.We found that root activity,total root length(TRL),total root surface area(TRSA),total root volume(TRV),total root diameter(TRD),root length density(RLD),and root surface area density(SAD)of Casuarina seedlings decreased under soil salt stress,but root tissue density(RTD)increased.Fungal inoculation markedly improved root activity and biomass in seedlings subjected to salt stress.The morphology and nutrient content of seedling roots under the same strain treatment were profoundly affected by changes in soil salinity.Compared with CK,the inoculation treatment significantly increased TRD,as well as root vigor,total phosphorus(TP)content at 3‰ and 6‰ salinity and TRV,TRSA and RSD at 9‰ salinity.At 3‰ salinity,TRL,RLD,root total nitrogen(TN)content of Z1 treatment was significantly higher than that of CK,and carbon to phosphorus(C/P)ratio was significantly lower than that of CK.The Z1+Y6 treatment also significantly increased TRSA,TRV,SAD,and root carbon to phosphorus(C/P)ratio at 3‰ and 6‰ salinity.At 9‰ salinity,the TP content and root vigor of Z1 and Z1+Y6 treatments were also significantly higher than those of CK.Based on the stepwise regression analysis,RLD was the main factor affecting seedling height,whereas root activity and TC content were the main factors affecting ground diameter.Path analysis showed that root tissue density,total root volume,total phosphorus content,and total root diameter were the main factors affecting biomass and that the direct effect of total roo

关 键 词：短枝木麻黄 土壤盐度 内生真菌 养分含量 根系形态 通径分析 

分 类 号：S792.93[农业科学—林木遗传育种]