心俞穴位埋线联合曲美他嗪对大鼠心肌梗死后能量代谢影响的研究  

Effects of acupoint catgut embedding at Xinshu combined with trimetazidine on myocardial energy metabolism after myocardial infarction in rats

作　　者：邰萱 黄小楼 韦雪兰 穆泉洁 杨珣 张婷 吴高鑫 TAI Xuan;HUANG Xiaolou;WEI Xuelan;MU Quanjie;YANG Xun;ZHANG Ting;WU Gaoxin(Acupuncture and Tuina College,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Department of Acupuncture and Moxibustion,the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China)

机构地区：[1]贵州中医药大学针灸推拿学院,贵州贵阳550025 [2]贵州中医药大学第一附属医院针灸科,贵州贵阳550001

出　　处：《海南医学院学报》2024年第19期1451-1458,共8页Journal of Hainan Medical University

基　　金：2024年度贵州省科技厅项目[黔科合基础-ZK(2024)一般388];2019年博士启动基金项目[贵中医博士启动(2019)138号];2022年贵州中医药大学研究生教育创新计划项目(YCXZRS202203)。

摘　　要：目的:观察心俞穴位埋线联合曲美他嗪对大鼠心肌梗死(MI)后能量代谢的影响。方法:以经典左前降支冠脉结扎术建立MI大鼠模型,将造模成功的大鼠随机分为模型组、埋线组、西药组、联合组,假手术组单开胸不结扎。术后第2天开始干预,并于术后7 d、28 d两个时间点观察。苏木精-伊红(HE)染色观察心肌组织病理形态;生化检测测定心肌组织ATP含量和血清中游离脂肪酸(FFA)浓度;Western blot法检测心肌组织AMPK、GLUT4蛋白表达。结果:与假手术组比较,术后7 d、28 d的模型组心肌细胞肿胀、排列紊乱并可见明显点状炎性细胞浸润,心肌细胞横截面积和血清FFA浓度增大、心肌ATP含量和AMPK、GLUT4蛋白表达降低(P<0.05)。术后7 d和28 d时,与模型组比较,3个治疗组的心肌细胞形态、排列及炎症细胞浸润程度均有不同程度的改善,心肌细胞横截面积和血清FFA浓度均不同程度减小、心肌ATP含量和AMPK、GLUT4蛋白表达均不同程度增加(P<0.05),其中联合组疗效比埋线组和西药组更明显(P<0.05)。与术后7 d的各治疗组比较,术后28 d各治疗组心肌ATP含量和AMPK、GLUT4蛋白表达均增大(均P<0.05),其中联合组的改善效应最明显(P<0.05)。结论:心俞穴位埋线联合曲美他嗪可进一步发挥对MI后心肌的保护作用,两者联合应用具有协同增效的作用,其作用机制可能是通过增加心肌组织AMPK和GLUT4蛋白的表达,可降低血清FFA浓度和增加心肌ATP含量来改善MI后心肌能量代谢紊乱,进而改善心肌细胞肥大,促进心肌损伤修复。Objective:To observe the impact of acupoint catgut embedding at Xinshu combined with trimetazidine on energy metabolism after myocardial infarction(MI)in rats.Methods:The MI rat model was constructed by the typical ligation of the left anterior descending branch of the coronary artery,and the successfully-modeled rats were randomly divided into the model group,the catgut embedding group,the western medicine group,and the combined group,another single thoracotomy without ligation was used as the sham operation group.The intervention was started on the second day after operation,and the observation was car⁃ried out at two different time points of 7 d and 28 d after operation.The pathological morphology of myocardial tissue was observed by hematoxylin-eosin(H&E)staining,the content of adenosine triphosphate(ATP)in myocardial tissue and the concentration of free fatty acid(FFA)in serum were determined by biochemical detection,and the expression of AMPK and GLUT4 in myocardi⁃al tissue was detected by western blot.Results:In model group at 7 d and 28 d after model construction,the myocardial cells were swollen and disorganized,and obvious punctate inflammatory cell infiltration were seen compared with the sham peration group.Cardiomyocyte cross-sectional area and serum FFA concentration were increased,and myocardial ATP content and expression of AMPK and GLUT4 were decreased(all P<0.05).At 7 d and 28 d after operation,compared with the model group,the morphol⁃ogy and arrangement of myocardial cells and the degree of inflammatory cell infiltration in the three treatment groups were im⁃proved to varying degrees,the cross-sectional area of myocardial cells and the concentration of serum FFA were decreased to vary⁃ing degrees,and the content of myocardial ATP and the expression of AMPK and GLUT4 protein were increased to varying de⁃grees(all P<0.05),and the curative effect of the combined group was more obvious than that of the catgut embedding group and the western medicine group(P<0.05).Compared with the trea

关 键 词：心俞穴位埋线 曲美他嗪 联合应用 心肌梗死 能量代谢 

分 类 号：R542.22[医药卫生—心血管疾病]